AATOM™ 680 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 645–695 nm wavelength range, which aligns with the 670 nm line of diode laser and the 676 nm line of Krypton-Ion laser. As a zwitterionic compound, AATOM™ 680 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make AATOM™ 680 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM™ 680 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

The DBCO derivative of AATOM™ 680 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This /derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, AATOM™ 680 PEG4 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.