AATOM™ 680 Tetrazine

AATOM™ 680 tetrazine is a TCO-reactive, far-red fluorescent dye with an excitation/emission of 679/696 nm that is used for FACS and FISH applications.

Click Chemistry Conjugation: Offers high yield and rapid, copper-free labeling of TCO-modified biomolecules
High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

The chemical structure of ATTO 680 tetrazine.

Datasheet

SDS

Catalog	Size	Price	Quantity
70297	1 mg	Price

Overview

AATOM™ 680 is a far-red fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 645–695 nm wavelength range, which aligns with the 670 nm line of diode laser and the 676 nm line of Krypton-Ion laser. As a zwitterionic compound, AATOM™ 680 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make AATOM™ 680 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, AATOM™ 680 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

AATOM™ 680 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers. This product is manufactured by AAT Bioquest and is not affiliated with ATTO-TEC GmbH.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AATOM™ 680 Tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.527 µL	607.637 µL	1.215 mL	6.076 mL	12.153 mL
5 mM	24.305 µL	121.527 µL	243.055 µL	1.215 mL	2.431 mL
10 mM	12.153 µL	60.764 µL	121.527 µL	607.637 µL	1.215 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	822.86 g/mol	=	mL	x	mM	=	mmol

References

View all 3 references: Citation Explorer

Avidin/Biotin Bioinspired Platform for Dual In Vivo 18F-PET/NIRF Molecular Imaging.

Authors:

Damont, Annelaure and Boisgard, Raphael and Dollé, Frédéric and Hollocou, Morgane and Kuhnast, Bertrand

Journal:

Bioconjugate chemistry (2017): 2524-2529

Oxazine dye-conjugated dna oligonucleotides: Förster resonance energy transfer in view of molecular dye-DNA interactions.

Authors:

Kupstat, Annette and Ritschel, Thomas and Kumke, Michael U

Journal:

Bioconjugate chemistry (2011): 2546-57

Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan.

Authors:

Marmé, Nicole and Knemeyer, Jens-Peter and Sauer, Markus and Wolfrum, Jürgen

Journal:

Bioconjugate chemistry (2003): 1133-9

Physical properties

Molecular weight	822.86
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.17
Extinction coefficient (cm ^-1 M ^-1)	125000
Excitation (nm)	679
Emission (nm)	696
Quantum yield	0.30

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on April 29, 2026