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ABY Dye qPCR Calibration Solution *10,000X*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 559 |
| Emission (nm) | 570 |
| Quantum yield | 0.581 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 559 | Emission (nm) 570 | Quantum yield 0.581 |
Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal. Since instruments can differ in fluorescence detection sensitivity, the concentrations needed to calibrate any dye, such as ABY, will depend on the device used. The AAT Bioquest ABY dye qPCR Calibration Solution supplied as a 10,000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems. Calibration and verification should be run every six months or as needed. Please refer to the instrument maintenance guide for complete instructions on use.
Platform
qPCR
| Recommended plate | PCR Microplate |
| Instrument specification(s) | ABY Filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.
Prepare a ABY calibration plate:
Prepare a ABY Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X ABY Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.
Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the ABY Dye qPCR Calibration working solution to all wells of a reaction plate.
Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For complete calibration instructions, refer to the maintenance guide for your instrument.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 559 |
| Emission (nm) | 570 |
| Quantum yield | 0.581 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
| NED Dye qPCR Calibration Solution *10,000X* | 545 | 567 | 740001 |
| FAM Dye qPCR Calibration Solution *10,000X* | 493 | 517 | 83000 |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| ROX Dye qPCR Calibration Solution *10,000X* | 578 | 595 | - |
| SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
| TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
| JUN Dye qPCR Calibration Solution *10,000X* | 610 | 628 | 1100001 |
| MP650 Dye qPCR Calibration Solution *10,000X* | 639 | 653 | 2500001 |
Show More (1) | |||
Images
Figure 1. ABI 7500 FAST system ABY dye spectrum.
References
View all 50 references: Citation Explorer
Bocaparvovirus in infants hospitalized in a neonatal intensive care unit in Midwest Brazil during the COVID-19 pandemic (2021-2022).
Authors: de Faria Gonçalves, Aline and Anjos, Déborah and de Oliveira Peixoto, Fernanda Aparecida and Franco, Fernanda Craveiro and Silva-Sales, Marcelle and Fiaccadori, Fabíola Souza and Porto, Pedro Soares and Souza, Menira
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2024): 315-322
Authors: de Faria Gonçalves, Aline and Anjos, Déborah and de Oliveira Peixoto, Fernanda Aparecida and Franco, Fernanda Craveiro and Silva-Sales, Marcelle and Fiaccadori, Fabíola Souza and Porto, Pedro Soares and Souza, Menira
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2024): 315-322
Evaluation of Extraction Methods to Detect Noroviruses in Ready-to-Eat Raw Milk Minas Artisanal Cheese.
Authors: Cantelli, Carina Pacheco and Silva, Marcio Roberto and Pimenta, Laís Marques and Tavares, Guilherme Caetano Lanzieri and Baduy, Gabriel Assad and Duch, André Almeida Santos and Menezes, Liliane Denize Miranda and Fialho, Alexandre Madi and Maranhão, Adriana Gonçalves and Fumian, Tulio Machado and Miagostovich, Marize Pereira and Leite, José Paulo Gagliardi
Journal: Food and environmental virology (2024)
Authors: Cantelli, Carina Pacheco and Silva, Marcio Roberto and Pimenta, Laís Marques and Tavares, Guilherme Caetano Lanzieri and Baduy, Gabriel Assad and Duch, André Almeida Santos and Menezes, Liliane Denize Miranda and Fialho, Alexandre Madi and Maranhão, Adriana Gonçalves and Fumian, Tulio Machado and Miagostovich, Marize Pereira and Leite, José Paulo Gagliardi
Journal: Food and environmental virology (2024)
Association of the rs1126616 and rs9138 Variants in the SPP1 Gene among Mexican Patients with Systemic Lupus Erythematosus and Lupus Nephritis.
Authors: Rivera-Cameras, Alicia and Gallegos-Arreola, Martha Patricia and Morán-Moguel, María Cristina and Salazar-Páramo, Mario and Alcaraz-López, Miriam Fabiola and Echeverría-González, Gustavo and Topete-Reyes, Jorge Fernando and Franco-Chávez, Sergio Adalberto and Dávalos-Rodríguez, Ingrid Patricia
Journal: International journal of molecular sciences (2024)
Authors: Rivera-Cameras, Alicia and Gallegos-Arreola, Martha Patricia and Morán-Moguel, María Cristina and Salazar-Páramo, Mario and Alcaraz-López, Miriam Fabiola and Echeverría-González, Gustavo and Topete-Reyes, Jorge Fernando and Franco-Chávez, Sergio Adalberto and Dávalos-Rodríguez, Ingrid Patricia
Journal: International journal of molecular sciences (2024)
A phase 2a open-label clinical trial to determine the effect of famciclovir on EBV activity as measured by EBV shedding in the saliva of patients with multiple sclerosis.
Authors: Dobson, Ruth and Holden, David and Vickaryous, Nicola and Bestwick, Jonathan and George, Katila and Sayali, Tatiana and Bianchi, Lucia and Wafa, Mohammad and Gold, Julian and Giovannoni, Gavin
Journal: Multiple sclerosis (Houndmills, Basingstoke, England) (2024): 63-70
Authors: Dobson, Ruth and Holden, David and Vickaryous, Nicola and Bestwick, Jonathan and George, Katila and Sayali, Tatiana and Bianchi, Lucia and Wafa, Mohammad and Gold, Julian and Giovannoni, Gavin
Journal: Multiple sclerosis (Houndmills, Basingstoke, England) (2024): 63-70
Species composition, infection rate and detection of resistant alleles in Anopheles funestus (Diptera: Culicidae) from Lare, a malaria hotspot district of Ethiopia.
Authors: Woyessa, Delelegn and Morou, Evangelia and Wipf, Nadja and Dada, Nsa and Mavridis, Konstantinos and Vontas, John and Yewhalaw, Delenasaw
Journal: Malaria journal (2023): 233
Authors: Woyessa, Delelegn and Morou, Evangelia and Wipf, Nadja and Dada, Nsa and Mavridis, Konstantinos and Vontas, John and Yewhalaw, Delenasaw
Journal: Malaria journal (2023): 233
Gene expression study of host-human T-cell leukaemia virus type 1 (HTLV-1) interactions: adult T-cell leukaemia/lymphoma (ATLL).
Authors: Rahimzada, Masooma and Nahavandi, Mehri and Saffari, Mona and Shafaei, Azam and Mosavat, Arman and Ahmadi Gezeldasht, Sanaz and Ariaee, Nazila and Valizadeh, Narges and Rahimi, Hossein and Rezaee, Seyed Abdolrahim and Derakhshan, Mohammad
Journal: Molecular biology reports (2023): 7479-7487
Authors: Rahimzada, Masooma and Nahavandi, Mehri and Saffari, Mona and Shafaei, Azam and Mosavat, Arman and Ahmadi Gezeldasht, Sanaz and Ariaee, Nazila and Valizadeh, Narges and Rahimi, Hossein and Rezaee, Seyed Abdolrahim and Derakhshan, Mohammad
Journal: Molecular biology reports (2023): 7479-7487
Comparative analysis of fish environmental DNA reveals higher sensitivity achieved through targeted sequence-based metabarcoding.
Authors: McCarthy, Avery and Rajabi, Hoda and McClenaghan, Beverly and Fahner, Nicole A and Porter, Emily and Singer, Gregory A C and Hajibabaei, Mehrdad
Journal: Molecular ecology resources (2023): 581-591
Authors: McCarthy, Avery and Rajabi, Hoda and McClenaghan, Beverly and Fahner, Nicole A and Porter, Emily and Singer, Gregory A C and Hajibabaei, Mehrdad
Journal: Molecular ecology resources (2023): 581-591
Human T-lymphotropic virus 2 (HTLV-2) prevalence of blood donors in the state of Pará, Brazil.
Authors: Correa, Lucas Pinheiro and da Costa Farias, Fatyene and Dos Santos Barile, Katarine Antonia and Palmeira, Maurício Koury and de Melo Amaral, Carlos Eduardo
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2023): 1745-1750
Authors: Correa, Lucas Pinheiro and da Costa Farias, Fatyene and Dos Santos Barile, Katarine Antonia and Palmeira, Maurício Koury and de Melo Amaral, Carlos Eduardo
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2023): 1745-1750
HTLV-1 Proviral Load Absolute RT-qPCR Development for Assessing on Clinical Outcomes in HAM/TSP Patients.
Authors: Ariaee, Nazila and Abbasnia, Shadi and Sabet, Faeze and Mirhossein, Ali and Ahmadi Ghezeldasht, Sanaz and Moshfegh, Mehdi and Boostani, Reza and Rezaee, Seyed Abdolrahim
Journal: Reports of biochemistry & molecular biology (2023): 393-402
Authors: Ariaee, Nazila and Abbasnia, Shadi and Sabet, Faeze and Mirhossein, Ali and Ahmadi Ghezeldasht, Sanaz and Moshfegh, Mehdi and Boostani, Reza and Rezaee, Seyed Abdolrahim
Journal: Reports of biochemistry & molecular biology (2023): 393-402
First report of 'Candidatus Phytoplasma pruni' associated with X-disease on sweet cherry (Prunus avium L.) in Canada.
Authors: Urbez-Torres, Jose Ramon and Sabaratnam, Siva and Acheampong, Susanna and Balcaen, Diana and Boule, Julie and Ghoshal, Basudev and Bennypaul, Harvinder and Thurston, Molly and Richardson, Tamara and Molnar, Cody and Harper, Scott
Journal: Plant disease (2023)
Authors: Urbez-Torres, Jose Ramon and Sabaratnam, Siva and Acheampong, Susanna and Balcaen, Diana and Boule, Julie and Ghoshal, Basudev and Bennypaul, Harvinder and Thurston, Molly and Richardson, Tamara and Molnar, Cody and Harper, Scott
Journal: Plant disease (2023)