Ac-DEVD-AFC *CAS 201608-14-2*

Ac-DEVD-AFC

CAS 201608-14-2

Ac-DEVD-AFC is a fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. The 7-amido-4-trifluoromethylcoumarin derivatives have better membrane permeability than the 7-amido-4-methylcoumarin derivatives.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13401	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-DEVD-AFC *CAS 201608-14-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.06 µL	685.298 µL	1.371 mL	6.853 mL	13.706 mL
5 mM	27.412 µL	137.06 µL	274.119 µL	1.371 mL	2.741 mL
10 mM	13.706 µL	68.53 µL	137.06 µL	685.298 µL	1.371 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	729.61 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Activation of mitophagy and proteasomal degradation confers resistance to developmental defects in postnatal skeletal muscle

Authors:

Rahman, Fasih A and Graham, Mackenzie Q and Truong, Alex and Quadrilatero, Joe

Journal:

Journal of Biomedical Science (2025): 77

Mitophagy is required to protect against excessive skeletal muscle atrophy following hindlimb immobilization

Authors:

Rahman, Fasih A and Graham, Mackenzie Q and Adam, Amanda M and Juracic, Emma S and Tupling, A Russell and Quadrilatero, Joe

Journal:

Journal of Biomedical Science (2025): 29

Modeling ferroptosis in human dopaminergic neurons: pitfalls and opportunities for neurodegeneration research

Authors:

Renner, Nadine and Sch{\"o}b, Franziska and Pape, Regina and Suciu, Ilinca and Spreng, Anna-Sophie and {\"U}ckert, Anna-Katharina and C{\"o}llen, Eike and Bovio, Federica and Chilian, Bruno and Bauer, Johannes and others,

Journal:

Redox Biology (2024): 103165

Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells

Authors:

Danshiitsoodol, Narandalai and Inoue, Yusuke and Sugimoto, Sachiko and Shakya, Shrijana and Noda, Masafumi and Sugiyama, Masanori

Journal:

Fermentation (2024): 197

Mitophagy regulates mitochondrial network signaling, oxidative stress, and apoptosis during myoblast differentiation

Authors:

Baechler, Brittany L and Bloemberg, Darin and Quadrilatero, Joe

Journal:

Autophagy (2019): 1606--1619

References

View all 67 references: Citation Explorer

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis

Authors:

Fennell M, Chan H, Wood A.

Journal:

J Biomol Screen (2006): 296

Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells

Authors:

Jiang DJ, Jia SJ, Dai Z, Li YJ.

Journal:

J Mol Cell Cardiol (2006): 529

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	729.61
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000 ¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	201608-14-2

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 21, 2025