Ac-DEVD-aminoluciferin

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Physical properties

Molecular weight	779.79
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	18000
Excitation (nm)	362
Emission (nm)	499

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

779.79

Extinction coefficient (cm ^-1 M ^-1)

18000

Excitation (nm)

362

Emission (nm)

499

Ac-DEVD-aminoluciferin is a luminogenic substrate that measures caspase-3 and -7 activities in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. Notably, caspases-3 and -7, target the DEVD motif in poly(ADP-ribose) polymerase (PARP), disrupting the repair capabilities of PARP and facilitating DNA fragmentation, a hallmark of apoptosis. When caspase-3/7 enzymes cleave the Ac-DEVD-aminoluciferin substrate, it releases aminoluciferin, which can react with luciferase to produce bioluminescence. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-3/7 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-DEVD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	128.24 µL	641.198 µL	1.282 mL	6.412 mL	12.824 mL
5 mM	25.648 µL	128.24 µL	256.479 µL	1.282 mL	2.565 mL
10 mM	12.824 µL	64.12 µL	128.24 µL	641.198 µL	1.282 mL