Ac-IETD-AFC *CAS 211990-57-7*

Ac-IETD-AFC

CAS 211990-57-7

Ac-IETD-AFC is a fluorogenic caspase-8/granzyme B substrate containing the acetyl (Ac) moiety. This substrate is hydrlyzed by caspase 8 to generate highly fluorescent 7-amido-4-trifluoromethylcoumarin (AFC).

Protocol

SDS

Catalog	Size	Price	Quantity
13410	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-IETD-AFC *CAS 211990-57-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	137.052 µL	685.26 µL	1.371 mL	6.853 mL	13.705 mL
5 mM	27.41 µL	137.052 µL	274.104 µL	1.371 mL	2.741 mL
10 mM	13.705 µL	68.526 µL	137.052 µL	685.26 µL	1.371 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	729.65 g/mol	=	mL	x	mM	=	mmol

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	729.65
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000 ¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	211990-57-7

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