Name: Ac-LEHD-AMC *CAS 292633-16-0*
Brand: AAT Bioquest
SKU: 13426
Price: 174 USD
Availability: InStock

Ac-LEHD-AMC

CAS 292633-16-0

Caspase 9 is a member of the CED-3 subfamily of the caspase family of cysteine proteases that play an essential role in the execution phase of apoptosis. These enzymes share a dominant primary specificity for cleaving bonds following aspartic acid residues. "Initiator" caspases (such as caspase 8) activate "effector" caspases, such as caspases 3 and 7. The effector caspases then cleave cellular substrates ultimately leading to the morphological changes of apoptosis. Ac-LEHD-AMC is a selective fluorogenic substrate for caspase 9. The caspase 9-induced hydrolysis of Ac-LEHD-AMC results in the release of AMC fluorophore that is detected using an excitation wavelength of ~365 nm and an emission wavelengh of ~450 nm. The assay can be run in the assay buffer consisting of 50 mM MES, pH 6.5, 10% PEG 8000, 0.1% CHAPS, 5 mM DTT, and 1 mM EDTA.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13426	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-LEHD-AMC *CAS 292633-16-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	140.505 µL	702.523 µL	1.405 mL	7.025 mL	14.05 mL
5 mM	28.101 µL	140.505 µL	281.009 µL	1.405 mL	2.81 mL
10 mM	14.05 µL	70.252 µL	140.505 µL	702.523 µL	1.405 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	711.72 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Structure-dependent induction of apoptosis by hepatotoxic pyrrolizidine alkaloids in the human hepatoma cell line HepaRG: Single versus repeated exposure

Authors:

Waizenegger, Julia and Braeuning, Albert and Templin, Markus and Lampen, Alfonso and Hessel-Pras, Stefanie

Journal:

Food and Chemical Toxicology (2018)

pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity

Authors:

Yu, Shaoxuan and Wang, Yanru and Zhang, Wentao and Zhang, Yuhuan and Zhu, Wenxin and Liu, Yingnan and Zhang, Daohong and Wang, Jianlong

Journal:

RSC Advances (2016): 72213--72223

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	711.72
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	292633-16-0

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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