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Ac-LEHD-AMC
CAS 292633-16-0
Caspase 9 is a member of the CED-3 subfamily of the caspase family of cysteine proteases that play an essential role in the execution phase of apoptosis. These enzymes share a dominant primary specificity for cleaving bonds following aspartic acid residues. "Initiator" caspases (such as caspase 8) activate "effector" caspases, such as caspases 3 and 7. The effector caspases then cleave cellular substrates ultimately leading to the morphological changes of apoptosis. Ac-LEHD-AMC is a selective fluorogenic substrate for caspase 9. The caspase 9-induced hydrolysis of Ac-LEHD-AMC results in the release of AMC fluorophore that is detected using an excitation wavelength of ~365 nm and an emission wavelengh of ~450 nm. The assay can be run in the assay buffer consisting of 50 mM MES, pH 6.5, 10% PEG 8000, 0.1% CHAPS, 5 mM DTT, and 1 mM EDTA.
Chemical structure for Ac-LEHD-AMC *CAS 292633-16-0*
Chemical structure for Ac-LEHD-AMC *CAS 292633-16-0*
CatalogSize
Price
Quantity
134265 mg
Price
 
Physical properties

Molecular weight711.72
SolventDMSO
Spectral properties

Excitation (nm)341
Emission (nm)441
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS292633-16-0
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Page updated on October 12, 2025