Ac-Leu-AMC

Ac-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase. Upon the hydrolysis by proteases, Leu-AMC releases the strongly fluorescent AMC fluorophore that can be readily detected with Ex/Em = 350/430 nm. Leucine aminopeptidase is an extracellular enzyme found abundantly in natural aquatic systems. It is one of the various enzymes used by bacteria for protein hydrolysis. Ac-L-Leucine-7-amido-4-methylcoumarin might be used as a substrate in fluorescence-based soil assays to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13444	1 mg	Price

References

View all 9 references: Citation Explorer

Skeletocutins M-Q: biologically active compounds from the fruiting bodies of the basidiomycete Skeletocutis sp. collected in Africa.

Authors:

Cheng, Tian and Chepkirui, Clara and Decock, Cony and Matasyoh, Josphat C and Stadler, Marc

Journal:

Beilstein journal of organic chemistry (2019): 2782-2789

The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction.

Authors:

Huang, Jian-Ming and Chang, Yao-Tsung and Lin, Wei-Chen

Journal:

Molecules (Basel, Switzerland) (2019)

Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani.

Authors:

Bhat, Saleem Yousuf and Dey, Arijit and Qureshi, Insaf A

Journal:

International journal of biological macromolecules (2018): 940-954

Discovery of a pyruvylated peptide-metabolizing enzyme using a fluorescent substrate-based protein discovery technique.

Authors:

Yoshioka, Kentaro and Komatsu, Toru and Hanaoka, Kenjiro and Ueno, Tasuku and Terai, Takuya and Nagano, Tetsuo and Urano, Yasuteru

Journal:

Chemical communications (Cambridge, England) (2016): 4377-80

A quantitative technique for determining proteases and their substrate specificities and pH optima in crude enzyme extracts.

Authors:

Budic, Maruska and Kidric, Marjetka and Meglic, Vladimir and Cigić, Blaz

Journal:

Analytical biochemistry (2009): 56-62

Physical properties

Molecular weight	330.38
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 5, 2025