Ac-LKKR-AMC

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Physical properties

Molecular weight	1084.99
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-LKKR-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	92.167 µL	460.834 µL	921.667 µL	4.608 mL	9.217 mL
5 mM	18.433 µL	92.167 µL	184.333 µL	921.667 µL	1.843 mL
10 mM	9.217 µL	46.083 µL	92.167 µL	460.834 µL	921.667 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	341
Emission (nm)	441

Product Family

Name	Excitation (nm)	Emission (nm)
Ac-DEVD-AMC CAS 169332-61-0	341	441
Ac-IETD-AMC	341	441
Ac-LEHD-AMC CAS 292633-16-0	341	441
Ac-Leu-AMC	341	441

Images

Figure 1. Ac-Leu-Lys-Lys-Arg-AMC is a fluorogenic aminopeptidase and endopeptidase substrate. The protease activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.

References

View all 50 references: Citation Explorer

Refolding of metacaspase 5 from Trypanosoma cruzi, structural characterization and the influence of c-terminal in protein recombinant production.
Authors: De Lima, Jhenifer Yonara and Santos, Marlon Dias Mariano Dos and Andreassa, Emanuella de Castro and Kurt, Louise Ulrich and Carvalho, Paulo Costa and De Souza, Tatiana de Arruda Campos Brasil
Journal: Protein expression and purification (2022): 106007

Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants.
Authors: Stael, Simon and Miller, Luke P and Fernández-Fernández, Álvaro D and Van Breusegem, Frank
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 127-137

Expression and Purification of the Type II Metacaspase from a Unicellular Green Alga Chlamydomonas reinhardtii.
Authors: Sabljić, Igor and Zou, Yong and Klemenčič, Marina and Funk, Christiane and Ståhlberg, Jerry and Bozhkov, Peter
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 13-20

Expression and Purification of the Type I Metacaspase from a Cryptophyte Guillardia theta , GtMCA-I.
Authors: Štrancar, Vida and van Midden, Katarina Petra and Klemenčič, Marina and Funk, Christiane
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 1-11

Dissecting The role of Plasmodium metacaspase-2 in malaria gametogenesis and sporogony.
Authors: Kumari, Vandana and Prasad, Kona Madhavinadha and Kalia, Inderjeet and Sindhu, Gagandeep and Dixit, Rajnikant and Rawat, Diwan S and Singh, O P and Singh, Agam P and Pandey, Kailash C
Journal: Emerging microbes & infections (2022): 938-955

Plasmodium vivax metacaspase 1 (PvMCA1) catalytic domain is conserved in field isolates from Brazilian Amazon.
Authors: de Souza, Hugo Amorim Dos Santos and Escafa, Victor Fernandes and Blanco, Carolina Moreira and Baptista, Bárbara de Oliveira and de Barros, Jenifer Peixoto and Riccio, Evelyn Ketty Pratt and Rodrigues, Aline Beatriz Mello and Melo, Gisely Cardoso de and Lacerda, Marcus Vinícius Guimarães de and de Souza, Rodrigo Medeiros and Lima-Junior, Josué da Costa and Guimarães, Ana Carolina Ramos and da Mota, Fabio Faria and da Silva, João Hermínio Martins and Daniel-Ribeiro, Cláudio Tadeu and Pratt-Riccio, Lilian Rose and Totino, Paulo Renato Rivas
Journal: Memorias do Instituto Oswaldo Cruz (2021): e200584

Targeting metacaspase-3 from Plasmodium falciparum towards antimalarial therapy: A combined approach of in-silico and in-vitro investigation.
Authors: Kumar, Bhumika and Mohammad, Taj and Amaduddin, and Hussain, Afzal and Islam, Asimul and Ahmad, Faizan and Alajmi, Mohamed F and Singh, Shailja and Pandey, Kailash C and Hassan, Md Imtaiyaz and Abid, Mohammad
Journal: Journal of biomolecular structure & dynamics (2021): 421-430

Suppression of Metacaspase- and Autophagy-Dependent Cell Death Improves Stress-Induced Microspore Embryogenesis in Brassica napus.
Authors: Berenguer, Eduardo and Minina, Elena A and Carneros, Elena and B R Ny, Ivett and Bozhkov, Peter V and Testillano, Pilar S
Journal: Plant & cell physiology (2021): 2097-2110

Biochemical Characterization of a Novel Redox-Regulated Metacaspase in a Marine Diatom.
Authors: Graff van Creveld, Shiri and Ben-Dor, Shifra and Mizrachi, Avia and Alcolombri, Uria and Hopes, Amanda and Mock, Thomas and Rosenwasser, Shilo and Vardi, Assaf
Journal: Frontiers in microbiology (2021): 688199

Screening and Identification of Metacaspase Inhibitors: Evaluation of Inhibition Mechanism and Trypanocidal Activity.
Authors: Pérez, Brian and Bouvier, León A and Cazzulo, Juan José and Agüero, Fernán and Salas-Sarduy, Emir and Alvarez, Vanina E
Journal: Antimicrobial agents and chemotherapy (2021)