Ac-Pro-Ala-Leu-AMC

The weakly fluorescent AMC substrates generate the bright blue fluorescent AMC product that has Ex/Em = 351/430 nm, and can be easily detected with a DAPI filter set. This AMC substrate is used for monitoring the protease activities of the proteasome. The most common form of the proteasome is known as the 26S proteasome that contains one 20S core particle structure and two 19S regulatory caps. All 20S particles consist of four stacked heptameric ring structures that are themselves composed of two different types of subunits; alpha subunits are structural in nature, whereas beta subunits are predominantly catalytic. The outer two rings in the stack consist of seven alpha subunits each, which serve as docking domains for the regulatory particles and the alpha subunits N-termini form a gate that blocks unregulated access of substrates to the interior cavity. The inner two rings each consist of seven beta subunits and contain the protease active sites that perform the proteolysis reactions.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13479	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-Pro-Ala-Leu-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	200.574 µL	1.003 mL	2.006 mL	10.029 mL	20.057 mL
5 mM	40.115 µL	200.574 µL	401.147 µL	2.006 mL	4.011 mL
10 mM	20.057 µL	100.287 µL	200.574 µL	1.003 mL	2.006 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	498.57 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Diabetogenic agent alloxan is a proteasome inhibitor

Authors:

Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue

Journal:

Biochemical and Biophysical Research Communications (2017): 400--406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations

Authors:

Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V

Journal:

Molecular & Cellular Proteomics (2016): 1962--1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts

Authors:

Ghosh, Rajeshwary and Goswami, Sumanta K and Feitoza, Luis Felipe BB and Hammock, Bruce and Gomes, Aldrin V

Journal:

International Journal of Cardiology (2016): 923--935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1

Authors:

Grimm, Stefanie and Ott, Christiane and Hörlacher, Melanie and Weber, Daniela and Höhn, Annika and Grune, Tilman

Journal:

Biochemical Journal (2012): 127--139

References

View all 36 references: Citation Explorer

Alveolar extracellular 20S proteasome in patients with acute respiratory distress syndrome

Authors:

Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.

Journal:

Am J Respir Crit Care Med (2009): 1098

Quinone reductase acts as a redox switch of the 20S yeast proteasome

Authors:

Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.

Journal:

EMBO Rep (2009): 65

Comparative expression analysis and characterization of 20S proteasomes in human intestinal tissues: The proteasome pattern as diagnostic tool for IBD patients

Authors:

Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.

Journal:

Inflamm Bowel Dis (2009): 526

Enzymatic properties of the 20S proteasome in wheat endosperm and its biochemical characteristics after seed imbibition

Authors:

Shi C, Rui Q, Xu LL.

Journal:

Plant Biol (Stuttg) (2009): 849

Toward a full characterization of the human 20S proteasome subunits and their isoforms by a combination of proteomic approaches

Authors:

Uttenweiler-Joseph S, Claverol S, Sylvius L, Bousquet-Dubouch MP, Burlet-Schiltz O, Monsarrat B.

Journal:

Methods Mol Biol (2008): 111

Physical properties

Molecular weight	498.57
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025