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Acridine orange
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 331.323 µL | 1.657 mL | 3.313 mL | 16.566 mL | 33.132 mL |
| 5 mM | 66.265 µL | 331.323 µL | 662.647 µL | 3.313 mL | 6.626 mL |
| 10 mM | 33.132 µL | 165.662 µL | 331.323 µL | 1.657 mL | 3.313 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Thiazole orange *CAS 107091-89-4* | 514 | 533 |
| Thiazole orange *10 mM in DMSO* | 514 | 533 |
| CytoTell™ Orange | 541 | 560 |
| LysoBrite™ Orange | 543 | 565 |
References
View all 56 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267
Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186
Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)
The use of acridine orange base (AOB) as molecular probe to characterize nonaqueous AOT reverse micelles
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356
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