Acridine orange *10 mg/mL solution in water*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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International | See distributors |
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 301.82 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 487 |
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Alternative formats
Acridine orange |
Overview | SDSProtocol |
CAS 65-61-2 | Molecular weight 301.82 | Absorbance (nm) 487 | Extinction coefficient (cm -1 M -1) 27000 | Excitation (nm) 490 | Emission (nm) 520 |
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange *10 mg/mL solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 331.323 µL | 1.657 mL | 3.313 mL | 16.566 mL | 33.132 mL |
5 mM | 66.265 µL | 331.323 µL | 662.647 µL | 3.313 mL | 6.626 mL |
10 mM | 33.132 µL | 165.662 µL | 331.323 µL | 1.657 mL | 3.313 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 487 |
Extinction coefficient (cm -1 M -1) | 27000 |
Excitation (nm) | 490 |
Emission (nm) | 520 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Thiazole orange *CAS 107091-89-4* | 514 | 533 |
Thiazole orange *10 mM in DMSO* | 514 | 533 |
CytoTell™ Orange | 541 | 560 |
LysoBrite™ Orange | 543 | 565 |
Citations
View all 2 citations: Citation Explorer
Prevention of tumor progression in inflammation-related carcinogenesis by anti-inflammatory and anti-mutagenic effects brought about by ingesting fermented brown rice and rice bran with Aspergillus oryzae (FBRA)
Authors: Nemoto, Hideyuki and Otake, Marina and Matsumoto, Takahiro and Izutsu, Runa and Jehung, Jumond P and Goto, Keisuke and Osaki, Mitsuhiko and Mayama, Masafumi and Shikanai, Masataka and Kobayashi, Hiroshi and others,
Journal: Journal of Functional Foods (2022): 104907
Authors: Nemoto, Hideyuki and Otake, Marina and Matsumoto, Takahiro and Izutsu, Runa and Jehung, Jumond P and Goto, Keisuke and Osaki, Mitsuhiko and Mayama, Masafumi and Shikanai, Masataka and Kobayashi, Hiroshi and others,
Journal: Journal of Functional Foods (2022): 104907
Label-free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion
Authors: Zhao, Lili and Zhao, Meng and Yang, Yu and Gu, Yajun and Zheng, Fang and Wang, Xuan and Zheng, Zhiyuan and Sun, Xuguo
Journal: Oncology Letters
Authors: Zhao, Lili and Zhao, Meng and Yang, Yu and Gu, Yajun and Zheng, Fang and Wang, Xuan and Zheng, Zhiyuan and Sun, Xuguo
Journal: Oncology Letters
References
View all 56 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Evaluation of the gram stain-acridine orange leukocyte cytospin test for diagnosis of catheter-related bloodstream infection in children on long-term parenteral nutrition
Authors: Ferroni A, Moumile K, Pasquier A, Berche P, Colomb V.
Journal: Eur J Clin Microbiol Infect Dis (2006): 199
Authors: Ferroni A, Moumile K, Pasquier A, Berche P, Colomb V.
Journal: Eur J Clin Microbiol Infect Dis (2006): 199
The interaction of acridine orange with anionic surfactant and its application to the determination of protein
Authors: Shi Y, Zheng WW, Wu XX, Zhou DH.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 1653
Authors: Shi Y, Zheng WW, Wu XX, Zhou DH.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 1653
Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres
Authors: Krolenko SA, Adamyan SY, Belyaeva TN, Mozhenok TP.
Journal: Cell Biol Int (2006): 933
Authors: Krolenko SA, Adamyan SY, Belyaeva TN, Mozhenok TP.
Journal: Cell Biol Int (2006): 933
Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223
Study on the formation and depolymerization of acridine orange dimer in acridine orange-sodium dodecyl benzene sulfonate-protein system
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Acridine orange used for photodynamic therapy accumulates in malignant musculoskeletal tumors depending on pH gradient
Authors: Matsubara T, Kusuzaki K, Matsumine A, Shintani K, Satonaka H, Uchida A.
Journal: Anticancer Res (2006): 187
Authors: Matsubara T, Kusuzaki K, Matsumine A, Shintani K, Satonaka H, Uchida A.
Journal: Anticancer Res (2006): 187
Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595