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Acridine orange
10 mg/mL solution in water
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.
Chemical structure for Acridine orange *10 mg/mL solution in water*
Chemical structure for Acridine orange *10 mg/mL solution in water*
CatalogSize
Price
Quantity
1750310 mL
Price
 
Physical properties

Molecular weight301.82
SolventWater
Spectral properties

Absorbance (nm)487
Extinction coefficient (cm -1 M -1)
27000
Excitation (nm)490
Emission (nm)520
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS65-61-2
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Page updated on November 1, 2024