Acridine orange 10 mg/mL solution in water

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Physical properties

Molecular weight	301.82
Solvent	Water

Spectral properties

Absorbance (nm)	487
Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	490
Emission (nm)	520

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Overview SDS Protocol

CAS

65-61-2

Molecular weight

301.82

Absorbance (nm)

487

Extinction coefficient (cm ^-1 M ^-1)

27000

Excitation (nm)

490

Emission (nm)

520

Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange *10 mg/mL solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	331.323 µL	1.657 mL	3.313 mL	16.566 mL	33.132 mL
5 mM	66.265 µL	331.323 µL	662.647 µL	3.313 mL	6.626 mL
10 mM	33.132 µL	165.662 µL	331.323 µL	1.657 mL	3.313 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)	487
Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	490
Emission (nm)	520

Product Family

Name	Excitation (nm)	Emission (nm)
Thiazole orange CAS 107091-89-4	514	533
Thiazole orange 10 mM in DMSO	514	533
CytoTell™ Orange	541	560
LysoBrite™ Orange	543	565

Images

Figure 1. Chemical structure for Acridine orange *10 mg/mL solution in water*

Citations

View all 2 citations: Citation Explorer

Prevention of tumor progression in inflammation-related carcinogenesis by anti-inflammatory and anti-mutagenic effects brought about by ingesting fermented brown rice and rice bran with Aspergillus oryzae (FBRA)
Authors: Nemoto, Hideyuki and Otake, Marina and Matsumoto, Takahiro and Izutsu, Runa and Jehung, Jumond P and Goto, Keisuke and Osaki, Mitsuhiko and Mayama, Masafumi and Shikanai, Masataka and Kobayashi, Hiroshi and others,
Journal: Journal of Functional Foods (2022): 104907

Label-free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion
Authors: Zhao, Lili and Zhao, Meng and Yang, Yu and Gu, Yajun and Zheng, Fang and Wang, Xuan and Zheng, Zhiyuan and Sun, Xuguo
Journal: Oncology Letters

References

View all 56 references: Citation Explorer

Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells
Authors: Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T.
Journal: J Photochem Photobiol B. (2006)

Re: The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate. (Mutagenesis, 20, 411-415, 2005)
Authors: Celik A, Ogenler O, Comelekoglu U.
Journal: Mutagenesis (2006): 267

Improvement of the acridine orange-protein-surfactant system for protein estimation based on aromatic ring stacking effect of sodium dodecyl benzene sulphonate
Authors: Wang F, Yang J, Wu X, Wang X, Guo C, Jia Z.
Journal: Luminescence (2006): 186

The use of acridine orange base (AOB) as molecular probe to characterize nonaqueous AOT reverse micelles
Authors: Falcone RD, Correa NM, Biasutti MA, Silber JJ.
Journal: J Colloid Interface Sci (2006): 356

Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation
Authors: Valentini A, Pucci D, Crispini A, Federici G, Bernardini S.
Journal: Chem Biol Interact (2006): 241

Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595

Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94

Acridine orange used for photodynamic therapy accumulates in malignant musculoskeletal tumors depending on pH gradient
Authors: Matsubara T, Kusuzaki K, Matsumine A, Shintani K, Satonaka H, Uchida A.
Journal: Anticancer Res (2006): 187

Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223

Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306