Acridinium Azide

Acridinium is a univalent radical derived from acridine. Acridinium azides are synthetic molecules known for their strong chemiluminescence, commonly used to label terminal alkynes on peptides, antibodies, and other biomolecules through copper-catalyzed click reactions. The chemiluminescence of acridinium maleimides is triggered by exposure to an alkaline hydrogen peroxide solution, facilitating their use in sensitive detection methodologies within immunoassays and various biological assays. For accurate signal detection in a chemiluminescence analyzer, a trigger solution containing (A) 0.1% H₂O₂ and 0.1 M HNO₃, and (B) 0.25 M NaOH and 2% Triton X-100 is recommended.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
26019	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	176.202 µL	881.011 µL	1.762 mL	8.81 mL	17.62 mL
5 mM	35.24 µL	176.202 µL	352.404 µL	1.762 mL	3.524 mL
10 mM	17.62 µL	88.101 µL	176.202 µL	881.011 µL	1.762 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	567.53 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Synthesis of 9-(substituted phenoxycarbonyl)-10-methylacridinium trifluoromethanesulfonates: Effects of the leaving group on chemiluminescent properties.

Authors:

Smith, Keith and Holland, Andy M and Woodhead, J Stuart and El-Hiti, Gamal A

Journal:

Luminescence : the journal of biological and chemical luminescence (2024): e4794

Development of a rapid and ultrasensitive magnetic chemiluminescence immunoassay for the detection of adiponectin and its clinical application.

Authors:

Li, Jiexia and Fan, Zhuqiao and Chen, Hanqi and Maria Da Costa, Ernestina and Zhou, Xiaomian and Yu, Nan

Journal:

Journal of pharmaceutical and biomedical analysis (2024): 115961

Acridinium-Based Chemiluminescent Receptor-Ligand Binding Assay for Protein/Peptide Hormones.

Authors:

Daubermann, André Guilherme and Dressano, Keini and de Oliveira Ceciliato, Paulo Henrique and Moura, Daniel S

Journal:

Methods in molecular biology (Clifton, N.J.) (2024): 253-263

Synthesis, structure elucidation, and chemiluminescent activity of new 9-substituted 10-(ω-(succinimidyloxycarbonyl)alkyl)acridinium esters.

Authors:

Smith, Keith and Mu, Xiaojing and Li, Zhaoqiang and Holland, Andy M and Woodhead, J Stuart and El-Hiti, Gamal A

Journal:

Luminescence : the journal of biological and chemical luminescence (2023): 487-496

Synthesis and chemiluminescent characteristics of two new acridinium esters.

Authors:

Smith, Keith and Mu, Xiaojing and Li, Zhaoqiang and Woodhead, J Stuart and El-Hiti, Gamal A

Journal:

Luminescence : the journal of biological and chemical luminescence (2023): 1857-1863

Physical properties

Molecular weight	567.53
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 13, 2025