Acridinium C2, NHS Ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 596.52 |
Solvent | DMSO |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Dyes by Functional Group
CAS 177332-37-5 | Molecular weight 596.52 |
The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins and other biological molecules can be used as sensitive detection methods in immunoassays and other biological detections. This acridinium C2 NHS can be readily used for labeling proteins (such as antibodies) and other amino-containing biomolecules. Compared to Lumiwox™ acridinium NHS ester (#26000), the acridinium label is released from the carrier (such as proteins) upon hydrogen peroxide activation. If you need the acridinium ester label remains bonded with the carrier, you would need use Lumiwox™ acridinium NHS ester instead. Trigger solution containing (A) 0.1% H2O2+ 0.1 M HNO31 and (B) 0.25 M NaOH + 2% Triton-100 can be used to detect the signal in chemiluminescence analyzer. For full detailed protocol, please refer to: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189797/pdf/micromachines-08-00149.pdf
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium C2, NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 167.639 µL | 838.195 µL | 1.676 mL | 8.382 mL | 16.764 mL |
5 mM | 33.528 µL | 167.639 µL | 335.278 µL | 1.676 mL | 3.353 mL |
10 mM | 16.764 µL | 83.819 µL | 167.639 µL | 838.195 µL | 1.676 mL |
Molarity calculator
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Citations
View all 26 citations: Citation Explorer
On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements
Authors: Krzyminski, K. K., Roshal, A. D., Rudnicki-Velasquez, P. B., Zamojc, K.
Journal: Luminescence (2019): ersion="1.0" encoding="UTF-8" ?>26005.enlEndN
Authors: Krzyminski, K. K., Roshal, A. D., Rudnicki-Velasquez, P. B., Zamojc, K.
Journal: Luminescence (2019): ersion="1.0" encoding="UTF-8" ?>26005.enlEndN
A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
Authors: Holec-Gasior, L., Ferra, B., Czechowska, J., Serdiuk, I. E., Krzyminski, K.
Journal: Diagn Microbiol Infect Dis (2018): 13-19
Authors: Holec-Gasior, L., Ferra, B., Czechowska, J., Serdiuk, I. E., Krzyminski, K.
Journal: Diagn Microbiol Infect Dis (2018): 13-19
Enhancement effect on the chemiluminescence of acridinium esters under neutral conditions
Authors: Nakazono, M., Nanbu, S.
Journal: Luminescence (2018): 345-348
Authors: Nakazono, M., Nanbu, S.
Journal: Luminescence (2018): 345-348
A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites
Authors: Natrajan, A., Wen, D.
Journal: Org Biomol Chem (2015): 2622-33
Authors: Natrajan, A., Wen, D.
Journal: Org Biomol Chem (2015): 2622-33
Chemiluminescence accompanied by the reaction of acridinium ester and manganese (II)
Authors: Ren, L., Cui, H.
Journal: Luminescence (2014): 929-32
Authors: Ren, L., Cui, H.
Journal: Luminescence (2014): 929-32
Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay
Authors: Arakawa, H., Tsuruoka, K., Ohno, K., Tajima, N., Nagano, H.
Journal: Luminescence (2014): 374-7
Authors: Arakawa, H., Tsuruoka, K., Ohno, K., Tajima, N., Nagano, H.
Journal: Luminescence (2014): 374-7
Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags
Authors: Natrajan, A., Wen, D., Sharpe, D.
Journal: Org Biomol Chem (2014): 3887-901
Authors: Natrajan, A., Wen, D., Sharpe, D.
Journal: Org Biomol Chem (2014): 3887-901
Quenching the chemiluminescence of acridinium ester by graphene oxide for label-free and homogeneous DNA detection
Authors: He, Y., Huang, G., Cui, H.
Journal: ACS Appl Mater Interfaces (2013): 11336-40
Authors: He, Y., Huang, G., Cui, H.
Journal: ACS Appl Mater Interfaces (2013): 11336-40
An efficient fluorescence sensor for superoxide with an acridinium ion-linked porphyrin triad
Authors: Kotani, H., Ohkubo, K., Crossley, M. J., Fukuzumi, S.
Journal: J Am Chem Soc (2011): 11092-5
Authors: Kotani, H., Ohkubo, K., Crossley, M. J., Fukuzumi, S.
Journal: J Am Chem Soc (2011): 11092-5
Effect of surfactants on the chemiluminescence of acridinium dimethylphenyl ester labels and their conjugates
Authors: Natrajan, A., Sharpe, D., Wen, D.
Journal: Org Biomol Chem (2011): 5092-103
Authors: Natrajan, A., Sharpe, D., Wen, D.
Journal: Org Biomol Chem (2011): 5092-103
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)