Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Acridinium C2, NHS Ester

Chemical structure for Acridinium C2, NHS Ester.
Chemical structure for Acridinium C2, NHS Ester.
Ordering information
Price ()
Catalog Number26015
Unit Size
Find Distributor
Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight596.52
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins and other biological molecules can be used as sensitive detection methods in immunoassays and other biological detections. This acridinium C2 NHS can be readily used for labeling proteins (such as antibodies) and other amino-containing biomolecules. Compared to Lumiwox™ acridinium NHS ester (#26000), the acridinium label is released from the carrier (such as proteins) upon hydrogen peroxide activation. If you need the acridinium ester label remains bonded with the carrier, you would need use Lumiwox™ acridinium NHS ester instead. Trigger solution containing (A) 0.1% H2O2+ 0.1 M HNO31 and (B) 0.25 M NaOH + 2% Triton-100 can be used to detect the signal in chemiluminescence analyzer. For full detailed protocol, please refer to:


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium C2, NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM167.639 µL838.195 µL1.676 mL8.382 mL16.764 mL
5 mM33.528 µL167.639 µL335.278 µL1.676 mL3.353 mL
10 mM16.764 µL83.819 µL167.639 µL838.195 µL1.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


View all 26 citations: Citation Explorer
On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements
Authors: Krzyminski, K. K., Roshal, A. D., Rudnicki-Velasquez, P. B., Zamojc, K.
Journal: Luminescence (2019): ersion="1.0" encoding="UTF-8" ?>26005.enlEndN
A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
Authors: Holec-Gasior, L., Ferra, B., Czechowska, J., Serdiuk, I. E., Krzyminski, K.
Journal: Diagn Microbiol Infect Dis (2018): 13-19
Enhancement effect on the chemiluminescence of acridinium esters under neutral conditions
Authors: Nakazono, M., Nanbu, S.
Journal: Luminescence (2018): 345-348
A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites
Authors: Natrajan, A., Wen, D.
Journal: Org Biomol Chem (2015): 2622-33
Chemiluminescence accompanied by the reaction of acridinium ester and manganese (II)
Authors: Ren, L., Cui, H.
Journal: Luminescence (2014): 929-32
Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay
Authors: Arakawa, H., Tsuruoka, K., Ohno, K., Tajima, N., Nagano, H.
Journal: Luminescence (2014): 374-7
Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags
Authors: Natrajan, A., Wen, D., Sharpe, D.
Journal: Org Biomol Chem (2014): 3887-901
Quenching the chemiluminescence of acridinium ester by graphene oxide for label-free and homogeneous DNA detection
Authors: He, Y., Huang, G., Cui, H.
Journal: ACS Appl Mater Interfaces (2013): 11336-40
An efficient fluorescence sensor for superoxide with an acridinium ion-linked porphyrin triad
Authors: Kotani, H., Ohkubo, K., Crossley, M. J., Fukuzumi, S.
Journal: J Am Chem Soc (2011): 11092-5
Effect of surfactants on the chemiluminescence of acridinium dimethylphenyl ester labels and their conjugates
Authors: Natrajan, A., Sharpe, D., Wen, D.
Journal: Org Biomol Chem (2011): 5092-103