Acridinium C2, NHS Ester

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	596.52
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

CAS

177332-37-5

Molecular weight

596.52

The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins and other biological molecules can be used as sensitive detection methods in immunoassays and other biological detections. This acridinium C2 NHS can be readily used for labeling proteins (such as antibodies) and other amino-containing biomolecules. Compared to Lumiwox™ acridinium NHS ester (#26000), the acridinium label is released from the carrier (such as proteins) upon hydrogen peroxide activation. If you need the acridinium ester label remains bonded with the carrier, you would need use Lumiwox™ acridinium NHS ester instead. Trigger solution containing (A) 0.1% H2O2+ 0.1 M HNO31 and (B) 0.25 M NaOH + 2% Triton-100 can be used to detect the signal in chemiluminescence analyzer. For full detailed protocol, please refer to: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189797/pdf/micromachines-08-00149.pdf

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Acridinium C2, NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	167.639 µL	838.195 µL	1.676 mL	8.382 mL	16.764 mL
5 mM	33.528 µL	167.639 µL	335.278 µL	1.676 mL	3.353 mL
10 mM	16.764 µL	83.819 µL	167.639 µL	838.195 µL	1.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for Acridinium C2, NHS Ester.

Citations

View all 26 citations: Citation Explorer

On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements
Authors: Krzyminski, K. K., Roshal, A. D., Rudnicki-Velasquez, P. B., Zamojc, K.
Journal: Luminescence (2019): ersion="1.0" encoding="UTF-8" ?>26005.enlEndN

A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
Authors: Holec-Gasior, L., Ferra, B., Czechowska, J., Serdiuk, I. E., Krzyminski, K.
Journal: Diagn Microbiol Infect Dis (2018): 13-19

Enhancement effect on the chemiluminescence of acridinium esters under neutral conditions
Authors: Nakazono, M., Nanbu, S.
Journal: Luminescence (2018): 345-348

A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites
Authors: Natrajan, A., Wen, D.
Journal: Org Biomol Chem (2015): 2622-33

Chemiluminescence accompanied by the reaction of acridinium ester and manganese (II)
Authors: Ren, L., Cui, H.
Journal: Luminescence (2014): 929-32

Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay
Authors: Arakawa, H., Tsuruoka, K., Ohno, K., Tajima, N., Nagano, H.
Journal: Luminescence (2014): 374-7

Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags
Authors: Natrajan, A., Wen, D., Sharpe, D.
Journal: Org Biomol Chem (2014): 3887-901

Quenching the chemiluminescence of acridinium ester by graphene oxide for label-free and homogeneous DNA detection
Authors: He, Y., Huang, G., Cui, H.
Journal: ACS Appl Mater Interfaces (2013): 11336-40

An efficient fluorescence sensor for superoxide with an acridinium ion-linked porphyrin triad
Authors: Kotani, H., Ohkubo, K., Crossley, M. J., Fukuzumi, S.
Journal: J Am Chem Soc (2011): 11092-5

Effect of surfactants on the chemiluminescence of acridinium dimethylphenyl ester labels and their conjugates
Authors: Natrajan, A., Sharpe, D., Wen, D.
Journal: Org Biomol Chem (2011): 5092-103