Acridinium C2, NHS Ester

The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins and other biological molecules can be used as sensitive detection methods in immunoassays and other biological detections. This acridinium C2 NHS can be readily used for labeling proteins (such as antibodies) and other amino-containing biomolecules. Compared to Lumiwox™ acridinium NHS ester (#26000), the acridinium label is released from the carrier (such as proteins) upon hydrogen peroxide activation. If you need the acridinium ester label remains bonded with the carrier, you would need use Lumiwox™ acridinium NHS ester instead. Trigger solution containing (A) 0.1% H2O2+ 0.1 M HNO31 and (B) 0.25 M NaOH + 2% Triton-100 can be used to detect the signal in chemiluminescence analyzer. For full detailed protocol, please refer to: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189797/pdf/micromachines-08-00149.pdf

Protocol

COA

Catalog	Size	Price	Quantity
26015	5 mg	Price
26016	1 mg	Price

Citations

View all 27 citations: Citation Explorer

ECM1 and ANXA1 in urinary extracellular vesicles serve as biomarkers for breast cancer

Authors:

Huang, Hai and Wan, Jingyu and Ao, Xudong and Qu, Shuang and Jia, Meng and Zhao, Keyu and Liang, Junqing and Zen, Ke and Liang, Hongwei

Journal:

Frontiers in Oncology (2024)

On the use of acridinium indicators for the chemiluminescent determination of the total antioxidant capacity of dietary supplements

Authors:

Krzyminski, K. K., Roshal, A. D., Rudnicki-Velasquez, P. B., Zamojc, K.

Journal:

Luminescence (2019): ersion="1.0" encoding="UTF-8" ?>26005.enlEndN

A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test

Authors:

Holec-Gasior, L., Ferra, B., Czechowska, J., Serdiuk, I. E., Krzyminski, K.

Journal:

Diagn Microbiol Infect Dis (2018): 13-19

Enhancement effect on the chemiluminescence of acridinium esters under neutral conditions

Authors:

Nakazono, M., Nanbu, S.

Journal:

Luminescence (2018): 345-348

A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites

Authors:

Natrajan, A., Wen, D.

Journal:

Org Biomol Chem (2015): 2622-33

Storage, safety and handling

Intended use

Research Use Only (RUO)

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