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Actin Lightning™ iFluor® 488 Phalloidin Conjugate
Actin Lightning™ iFluor® 488 Phalloidin Conjugate is a green fluorescent high-affinity phalloidin probe designed for bright F-actin staining with enhanced signal stability.
  • High-affinity staining: Designed to preserve strong phalloidin binding to filamentous actin
  • Long-lasting signal: Supports F-actin imaging for around one month after staining under appropriate conditions
  • Green fluorescence: Labeled with iFluor® 488, with excitation/emission maxima of 491/516 nm
  • Imaging applications: Suitable for fluorescence microscopy analysis of cell morphology, cytoskeletal organization, and actin filament distribution
  • Enhanced staining performance: Actin Lightning conjugates provide superior performance compared to the regular phalloidin conjugates and other branded actin stains
Fluorescence image of HeLa cells stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate and Nuclear Blue™ DCS1. Cells were fixed with 4% formaldehyde, permeabilized with 0.2% Triton™ X-100, then stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate (Cat. #22710) for F-actin detection and counterstained with Nuclear Blue™ DCS1 (Cat. #17548). Images were acquired using FITC and DAPI filter sets and overlaid to show F-actin in green and nuclei in blue.
Fluorescence image of HeLa cells stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate and Nuclear Blue™ DCS1. Cells were fixed with 4% formaldehyde, permeabilized with 0.2% Triton™ X-100, then stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate (Cat. #22710) for F-actin detection and counterstained with Nuclear Blue™ DCS1 (Cat. #17548). Images were acquired using FITC and DAPI filter sets and overlaid to show F-actin in green and nuclei in blue.
CatalogSize
Price
Quantity
22710100 Tests
Price
 
Physical properties

SolventDMSO
Spectral properties

Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on June 24, 2026