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Actin Lightning™ iFluor® 488 Phalloidin Conjugate
Actin Lightning™ iFluor® 488 Phalloidin Conjugate is a green fluorescent high-affinity phalloidin probe designed for bright F-actin staining with enhanced signal stability.
  • High-affinity staining: Designed to preserve strong phalloidin binding to filamentous actin
  • Long-lasting signal: Supports F-actin imaging for around one month after staining under appropriate conditions
  • Green fluorescence: Labeled with iFluor® 488, with excitation/emission maxima of 491/516 nm
  • Imaging applications: Suitable for fluorescence microscopy analysis of cell morphology, cytoskeletal organization, and actin filament distribution
  • Enhanced staining performance: Actin Lightning conjugates provide superior performance compared to the regular phalloidin conjugates and other branded actin stains
Fluorescence image of HeLa cells stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate and Nuclear Blue™ DCS1. Cells were fixed with 4% formaldehyde, permeabilized with 0.2% Triton™ X-100, then stained with Actin Lightning™ iFluor® 488 Phalloidin Conjugate (Cat. #22710) for F-actin detection and counterstained with Nuclear Blue™ DCS1 (Cat. #17548). Images were acquired using FITC and DAPI filter sets and overlaid to show F-actin in green and nuclei in blue.
CatalogSize
Price
Quantity
22710100 Tests
Price
 
Physical properties

Molecular weight1451.45
SolventDMSO
Spectral properties

Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Usage and storage

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on August 11, 2026