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Actin Lightning™ iFluor® 647 Phalloidin Conjugate
Actin Lightning™ iFluor® 647 Phalloidin Conjugate is a far-red fluorescent high-affinity phalloidin conjugate designed for bright F-actin staining with enhanced signal stability.
  • High-affinity staining: Designed to preserve strong phalloidin binding to filamentous actin
  • Long-lasting signal: Supports F-actin imaging for around one month after staining under appropriate storage and mounting conditions
  • Far-red fluorescence: Labeled with iFluor® 647, with excitation/emission maxima of 656/670 nm
  • Imaging applications: Suitable for fluorescence microscopy analysis of cell morphology, cytoskeletal organization, and actin filament distribution
  • Enhanced staining performance: Actin Lightning conjugates provide superior performance compared to the regular phalloidin conjugates and other branded actin stains
Fluorescence image of CPA 47 cells stained with Actin Lightning™ iFluor® 647 Phalloidin Conjugate and Nuclear Blue™ DCS1. Cells were fixed with 4% formaldehyde, permeabilized with 0.2% Triton™ X-100, then stained with Actin Lightning™ iFluor® 647 Phalloidin Conjugate (Cat. #22719) for F-actin detection and counterstained with Nuclear Blue™ DCS1 (Cat. #17548). Images were acquired using Cy5 and DAPI filter sets and overlaid to show F-actin in red and nuclei in blue.
CatalogSize
Price
Quantity
22719100 Tests
Price
 
Physical properties

SolventDMSO
Spectral properties

Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Usage and storage

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings

Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
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Page updated on July 22, 2026