Name: Actin Lightning™ LC Red *Cell-Permeable*
Brand: AAT Bioquest
SKU: 22705
Price: 195 USD
Availability: InStock

Actin Lightning™ LC Red

Cell-Permeable

Actin Lightning™ LC Red is a live-cell compatible fluorescent probe designed for direct staining and visualization of filamentous actin (F-actin) in living cells.

Live-cell compatible: Enables direct visualization of actin filaments in living cells without fixation
Efflux pump independent: Does not require efflux inhibitors such as probenecid or verapamil during staining
Fixable staining: Fluorescent labeling is retained after chemical fixation for downstream multiplex workflows
Multipurpose use: Suitable for monitoring cytoskeletal organization, cell morphology, and actin dynamics in fluorescence microscopy

Super-resolution fluorescence image of live HeLa cells stained with Actin Lightning™ LC Red and MitoLite™ Green FM. Live HeLa cells were labeled with Actin Lightning™ LC Red (Cat. #22705) for F-actin staining and MitoLite™ Green FM (Cat. #22695) for mitochondrial staining. Images were acquired on the CSR Biotech MI-SIM microscopy system and reconstructed using 3D-SIM Wiener reconstruction. The merged image shows F-actin in magenta and mitochondria in green.

Image credit: Images were generated by CSR Biotech on their MI-SIM microscopy system using Actin Lightning™ LC Red and MitoLite™ Green FM from AAT Bioquest.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22705	100 Tests	Price

Overview

Actin Lightning™ LC Red is a membrane-permeable fluorescent probe developed for staining filamentous actin (F-actin) in live cells. The dye penetrates intact cell membranes and binds selectively to actin filaments, enabling visualization of cytoskeletal architecture in living cells without the need for fixation during staining. This property allows researchers to monitor actin organization and cellular morphology under physiological conditions using fluorescence microscopy.

Unlike other commercially available live-cell actin probes that require efflux pump inhibitors to maintain intracellular signal, Actin Lightning™ LC Red does not require additives such as probenecid or verapamil. The probe can also be used in workflows where cells are subsequently fixed, as the staining signal is retained after fixation. These characteristics support flexible imaging workflows for studying actin dynamics, cytoskeletal rearrangement, and cell morphology in live-cell multiplex experiments.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actin Lightning™ LC Red *Cell-Permeable* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.488 µL	482.439 µL	964.878 µL	4.824 mL	9.649 mL
5 mM	19.298 µL	96.488 µL	192.976 µL	964.878 µL	1.93 mL
10 mM	9.649 µL	48.244 µL	96.488 µL	482.439 µL	964.878 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1036.4 g/mol	=	mL	x	mM	=	mmol

References

View all 10 references: Citation Explorer

SiR-XActin: A Fluorescent Probe for Imaging Actin Dynamics in Live Cells.

Authors:

Nasufovic, Veselin and Kompa, Julian and Lindamood, Halli L and Blümke, Merle and Dibsy, Rayane and Koch, Birgit and Levario-Diaz, Victoria and Weber, Katharina and Maager, Marlene and Nomerotskaia, Ekaterina and Echard, Arnaud and Cavalcanti-Adam, Elisabetta Ada and Vitriol, Eric A and Arndt, Hans-Dieter and Johnsson, Kai

Journal:

Angewandte Chemie (International ed. in English) (2025): e202509285

mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells.

Authors:

Hatano, Tomoyuki and Lim, Tzer Chyn and Billault-Chaumartin, Ingrid and Dhar, Anubhav and Gu, Ying and Massam-Wu, Teresa and Scott, William and Adishesha, Sushmitha and Chapa-Y-Lazo, Bernardo and Springall, Luke and Sivashanmugam, Lavanya and Mishima, Masanori and Martin, Sophie G and Oliferenko, Snezhana and Palani, Saravanan and Balasubramanian, Mohan K

Journal:

Journal of cell science (2022)

Synthesis of Fluorescent Jasplakinolide Analogues for Live-Cell STED Microscopy of Actin.

Authors:

Belov, Vladimir N and Stoldt, Stefan and Rüttger, Franziska and John, Michael and Seikowski, Jan and Schimpfhauser, Jens and Hell, Stefan W

Journal:

The Journal of organic chemistry (2020): 7267-7275

Using carboxyfluorescein diacetate succinimidyl ester to monitor intracellular protein glycation.

Authors:

Boucher, Julie and Simard, Élie and Froehlich, Ulrike and D'Orléans-Juste, Pedro and Grandbois, Michel

Journal:

Analytical biochemistry (2015): 73-81

STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons.

Authors:

D'Este, Elisa and Kamin, Dirk and Göttfert, Fabian and El-Hady, Ahmed and Hell, Stefan W

Journal:

Cell reports (2015): 1246-51

Physical properties

Molecular weight	1036.4
Solvent	DMSO

Spectral properties

Excitation (nm)	647
Emission (nm)	665

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 22, 2026