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Actinomycin D

Chemical structure for Actinomycin D
Chemical structure for Actinomycin D
Ordering information
Price ()
Catalog Number17505
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1255.42
SolventDMSO
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
50-76-0
Molecular weight
1255.42
Actinomycin is a polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. It binds to DNA duplexes, thereby interfering with the action of enzymes engaged in replication and transcription. Actinomycin D is one of the old chemotherapy drugs which have been used in therapy for many years.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actinomycin D to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM79.655 µL398.273 µL796.546 µL3.983 mL7.965 mL
5 mM15.931 µL79.655 µL159.309 µL796.546 µL1.593 mL
10 mM7.965 µL39.827 µL79.655 µL398.273 µL796.546 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
D-Aminoluciferin36249918000

Citations


View all 4 citations: Citation Explorer
CircRNA\_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p
Authors: Liu, Wei and Lei, Lei and Liu, Xiaoying and Ye, Suiyan
Journal: Genes \& Genomics (2021): 653--667
Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis
Authors: Dong, Wenjie and Zhao, Lei and Zhang, Shiyang and Zhang, Shijie and Si, Hongyun
Journal: World Journal of Surgical Oncology (2021): 1--13
Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation
Authors: Xie, Mengyan and Yu, Tao and Jing, Xinming and Ma, Ling and Fan, Yu and Yang, Fengming and Ma, Pei and Jiang, Huning and Wu, Xi and Shu, Yongqian and others,
Journal: Molecular Cancer (2020): 1--22
NapM, a new nucleoid-associated protein, broadly regulates gene expression and affects mycobacterial resistance to anti-tuberculosis drugs
Authors: Liu, Yu and Wang, Hongyang and Cui, Tao and Zhou, Xiling and Jia, Yanxia and Zhang, Hua and He, Zheng-Guo
Journal: Molecular microbiology (2016): 167--181

References


View all 66 references: Citation Explorer
Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods
Authors: Vives M, Tauler R, Eritja R, Gargallo R.
Journal: Anal Bioanal Chem. (2006)
Treatment of wilms tumor relapsing after initial treatment with vincristine and actinomycin D: A report from the National Wilms Tumor Study Group
Authors: Green DM, Cotton CA, Malogolowkin M, Breslow NE, Perlman E, Miser J, Ritchey ML, Thomas PR, Grundy PE, D'Angio GJ, Beckwith JB, Shamberger RC, Haase GM, Donaldson M, Weetman R, Coppes MJ, Shearer P, Coccia P, Kletzel M, Macklis R, Tomlinson G, Huff V, Newbury R, Weeks D.
Journal: Pediatr Blood Cancer. (2006)
A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of actinomycin-D and vincristine in children with cancer
Authors: Skolnik JM, Barrett JS, Shi H, Adamson PC.
Journal: Cancer Chemother Pharmacol (2006): 458
Soluble TRAIL gene and actinomycin D synergistically suppressed multiple metastasis of TRAIL-resistant colon cancer in the liver
Authors: Ishii M, Iwai M, Harada Y, Kishida T, Asada H, Shin-Ya M, Itoh Y, Imanishi J, Okanoue T, Mazda O.
Journal: Cancer Lett. (2006)
Results with EMA/CO (etoposide, methotrexate, actinomycin D, cyclophosphamide, vincristine) chemotherapy in gestational trophoblastic neoplasia
Authors: Turan T, Karacay O, Tulunay G, Boran N, Koc S, Bozok S, Kose MF.
Journal: Int J Gynecol Cancer (2006): 1432
Fluorescent intercalator displacement analyses of DNA binding by the peptide-derived natural products netropsin, actinomycin, and bleomycin
Authors: Lewis MA, Long EC.
Journal: Bioorg Med Chem (2006): 3481
Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer
Authors: Kurosawa K, Bui VP, VanEssendelft JL, Willis LB, Lessard PA, Ghiviriga I, Samb and an TG, Rha CK, Sinskey AJ.
Journal: Appl Microbiol Biotechnol (2006): 145
Binding of actinomycin C1 (D) and actinomin to base-modified oligonucleotide duplexes with parallel chain orientation
Authors: Li H, Peng X, Leonard P, Seela F.
Journal: Bioorg Med Chem (2006): 4089
Tri-iodo thyronine regulates antioxidant enzyme activities in different cell fractions through a mechanism sensitive to actinomycin D in a teleost, Anabas testudineus (Bloch)
Authors: Saumya SM, Sreejith P, Vijayasree AS, Divya L, Manju M, Oommen OV.
Journal: Indian J Exp Biol (2006): 640
Expression of cell kinetics and death during monocyte-macrophage differentiation: effects of Actinomycin D and Vinblastine treatments
Authors: Spano A, Monaco G, Barni S, Sciola L.
Journal: Histochem Cell Biol. (2006)