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Actinomycin D

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Physical properties
Molecular weight1255.42
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Actinomycin is a polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. It binds to DNA duplexes, thereby interfering with the action of enzymes engaged in replication and transcription. Actinomycin D is one of the old chemotherapy drugs which have been used in therapy for many years.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actinomycin D to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM79.655 µL398.273 µL796.546 µL3.983 mL7.965 mL
5 mM15.931 µL79.655 µL159.309 µL796.546 µL1.593 mL
10 mM7.965 µL39.827 µL79.655 µL398.273 µL796.546 µL

Molarity calculator

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NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)



View all 10 citations: Citation Explorer
N6-methyladenosine-regulated ADIRF impairs lung adenocarcinoma metastasis and serves as a potential prognostic biomarker
Authors: Teng, Yin and Zhao, Xiaohan and Xi, Yang and Fu, Ninghua
Journal: Cancer Biology \& Therapy (2023): 2249173
METTL3-mediated m6A methylation orchestrates mRNA stability and dsRNA contents to equilibrate $\gamma$$\delta$ T1 and $\gamma$$\delta$ T17 cells
Authors: Xiao, Zhiqiang and Wang, Shanshan and Tian, Yixia and Lv, Wenkai and Sheng, Hao and Zhan, Mingjie and Huang, Qiongxiao and Zhang, Zhanpeng and Zhu, Leqing and Zhu, Chuyun and others,
Journal: Cell Reports (2023)
MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability
Authors: Liu, Jin-Xin and Ao-Nan, Chen and Yu, Qihong and Shi, Ke-Tai and Liu, Yi-Bo and Guo, Cui-Lian and Wang, Zhe-Zheng and Yao, Yin and Pan, Li and Lu, Xiang and others,
Journal: JCI insight (2023)
METTL3 regulates N6-methyladenosine modification of ANGPTL3 mRNA and potentiates malignant progression of stomach adenocarcinoma
Authors: Zhang, Zhijin and Fu, Jun and Zhang, Yuhao and Qin, Xianju and Wang, Yuexia and Xing, Chungen
Journal: BMC gastroenterology (2023): 1--14
Knockdown of METTL16 disrupts learning and memory by reducing the stability of MAT2A mRNA
Authors: Zhang, Runjiao and Zhang, Yizhou and Guo, Fangzhen and Huang, Guannan and Zhao, Yan and Chen, Bingyu and Wang, Chang and Cui, Chengran and Shi, Yichun and Li, Sha and others,
Journal: Cell death discovery (2022): 1--14
Exosomal circZFR promotes colorectal cancer progression via interacting with BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
Authors: Chen, Jiaxin and Xu, Jianbin and Wang, Huijuan and Chen, Engeng and Meng, Qing and Wang, Jiawei and Zhou, Wei and Shan, Ge and Ju, Zhenyu and Song, Zhangfa
Journal: (2022)
CircRNA\_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p
Authors: Liu, Wei and Lei, Lei and Liu, Xiaoying and Ye, Suiyan
Journal: Genes \& Genomics (2021): 653--667
Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis
Authors: Dong, Wenjie and Zhao, Lei and Zhang, Shiyang and Zhang, Shijie and Si, Hongyun
Journal: World Journal of Surgical Oncology (2021): 1--13
Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation
Authors: Xie, Mengyan and Yu, Tao and Jing, Xinming and Ma, Ling and Fan, Yu and Yang, Fengming and Ma, Pei and Jiang, Huning and Wu, Xi and Shu, Yongqian and others,
Journal: Molecular Cancer (2020): 1--22
NapM, a new nucleoid-associated protein, broadly regulates gene expression and affects mycobacterial resistance to anti-tuberculosis drugs
Authors: Liu, Yu and Wang, Hongyang and Cui, Tao and Zhou, Xiling and Jia, Yanxia and Zhang, Hua and He, Zheng-Guo
Journal: Molecular microbiology (2016): 167--181


View all 66 references: Citation Explorer
Spectroscopic study of the interaction of actinomycin D with oligonucleotides carrying the central base sequences -XGCY- and -XGGCCY- using multivariate methods
Authors: Vives M, Tauler R, Eritja R, Gargallo R.
Journal: Anal Bioanal Chem. (2006)
Treatment of wilms tumor relapsing after initial treatment with vincristine and actinomycin D: A report from the National Wilms Tumor Study Group
Authors: Green DM, Cotton CA, Malogolowkin M, Breslow NE, Perlman E, Miser J, Ritchey ML, Thomas PR, Grundy PE, D'Angio GJ, Beckwith JB, Shamberger RC, Haase GM, Donaldson M, Weetman R, Coppes MJ, Shearer P, Coccia P, Kletzel M, Macklis R, Tomlinson G, Huff V, Newbury R, Weeks D.
Journal: Pediatr Blood Cancer. (2006)
A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of actinomycin-D and vincristine in children with cancer
Authors: Skolnik JM, Barrett JS, Shi H, Adamson PC.
Journal: Cancer Chemother Pharmacol (2006): 458
Soluble TRAIL gene and actinomycin D synergistically suppressed multiple metastasis of TRAIL-resistant colon cancer in the liver
Authors: Ishii M, Iwai M, Harada Y, Kishida T, Asada H, Shin-Ya M, Itoh Y, Imanishi J, Okanoue T, Mazda O.
Journal: Cancer Lett. (2006)
Results with EMA/CO (etoposide, methotrexate, actinomycin D, cyclophosphamide, vincristine) chemotherapy in gestational trophoblastic neoplasia
Authors: Turan T, Karacay O, Tulunay G, Boran N, Koc S, Bozok S, Kose MF.
Journal: Int J Gynecol Cancer (2006): 1432
Fluorescent intercalator displacement analyses of DNA binding by the peptide-derived natural products netropsin, actinomycin, and bleomycin
Authors: Lewis MA, Long EC.
Journal: Bioorg Med Chem (2006): 3481
Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer
Authors: Kurosawa K, Bui VP, VanEssendelft JL, Willis LB, Lessard PA, Ghiviriga I, Samb and an TG, Rha CK, Sinskey AJ.
Journal: Appl Microbiol Biotechnol (2006): 145
Binding of actinomycin C1 (D) and actinomin to base-modified oligonucleotide duplexes with parallel chain orientation
Authors: Li H, Peng X, Leonard P, Seela F.
Journal: Bioorg Med Chem (2006): 4089
Tri-iodo thyronine regulates antioxidant enzyme activities in different cell fractions through a mechanism sensitive to actinomycin D in a teleost, Anabas testudineus (Bloch)
Authors: Saumya SM, Sreejith P, Vijayasree AS, Divya L, Manju M, Oommen OV.
Journal: Indian J Exp Biol (2006): 640
Expression of cell kinetics and death during monocyte-macrophage differentiation: effects of Actinomycin D and Vinblastine treatments
Authors: Spano A, Monaco G, Barni S, Sciola L.
Journal: Histochem Cell Biol. (2006)