AAT Bioquest

Actinomycin D

Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight1255.42
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Actinomycin is a polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. It binds to DNA duplexes, thereby interfering with the action of enzymes engaged in replication and transcription. Actinomycin D is one of the old chemotherapy drugs which have been used in therapy for many years.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actinomycin D to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM79.655 µL398.273 µL796.546 µL3.983 mL7.965 mL
5 mM15.931 µL79.655 µL159.309 µL796.546 µL1.593 mL
10 mM7.965 µL39.827 µL79.655 µL398.273 µL796.546 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)



View all 13 citations: Citation Explorer
METTL14 inhibits the malignant processes of gastric cancer cells by promoting N6-methyladenosine (m6A) methylation of TAF10
Authors: Zhao, Xin and Lu, Jingfen and Wu, Weimin and Li, Jiahui
Journal: Heliyon (2024)
Circ\_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
Authors: Qian, Wenjie and Mei, Kai and Zhu, Lei and Chu, Ying and Lv, Jinpeng and Yun, Changjun
Journal: Journal of Orthopaedic Surgery and Research (2024): 241
HBV DNA polymerase upregulates the transcription of PD-L1 and suppresses T cell activity in hepatocellular carcinoma
Authors: Jia, Yan and Zhao, Jianing and Wang, Chunqing and Meng, Jing and Zhao, Liqing and Yang, Hongwei and Zhao, Xiaoqing
Journal: Journal of Translational Medicine (2024): 1--17
N6-methyladenosine-regulated ADIRF impairs lung adenocarcinoma metastasis and serves as a potential prognostic biomarker
Authors: Teng, Yin and Zhao, Xiaohan and Xi, Yang and Fu, Ninghua
Journal: Cancer Biology \& Therapy (2023): 2249173
METTL3-mediated m6A methylation orchestrates mRNA stability and dsRNA contents to equilibrate $\gamma$$\delta$ T1 and $\gamma$$\delta$ T17 cells
Authors: Xiao, Zhiqiang and Wang, Shanshan and Tian, Yixia and Lv, Wenkai and Sheng, Hao and Zhan, Mingjie and Huang, Qiongxiao and Zhang, Zhanpeng and Zhu, Leqing and Zhu, Chuyun and others,
Journal: Cell Reports (2023)
METTL3 regulates N6-methyladenosine modification of ANGPTL3 mRNA and potentiates malignant progression of stomach adenocarcinoma
Authors: Zhang, Zhijin and Fu, Jun and Zhang, Yuhao and Qin, Xianju and Wang, Yuexia and Xing, Chungen
Journal: BMC gastroenterology (2023): 1--14
MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability
Authors: Liu, Jin-Xin and Ao-Nan, Chen and Yu, Qihong and Shi, Ke-Tai and Liu, Yi-Bo and Guo, Cui-Lian and Wang, Zhe-Zheng and Yao, Yin and Pan, Li and Lu, Xiang and others,
Journal: JCI insight (2023)
Knockdown of METTL16 disrupts learning and memory by reducing the stability of MAT2A mRNA
Authors: Zhang, Runjiao and Zhang, Yizhou and Guo, Fangzhen and Huang, Guannan and Zhao, Yan and Chen, Bingyu and Wang, Chang and Cui, Chengran and Shi, Yichun and Li, Sha and others,
Journal: Cell death discovery (2022): 1--14
Exosomal circZFR promotes colorectal cancer progression via interacting with BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
Authors: Chen, Jiaxin and Xu, Jianbin and Wang, Huijuan and Chen, Engeng and Meng, Qing and Wang, Jiawei and Zhou, Wei and Shan, Ge and Ju, Zhenyu and Song, Zhangfa
Journal: (2022)
CircRNA\_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p
Authors: Liu, Wei and Lei, Lei and Liu, Xiaoying and Ye, Suiyan
Journal: Genes \& Genomics (2021): 653--667


View all 66 references: Citation Explorer
Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer
Authors: Kurosawa K, Bui VP, VanEssendelft JL, Willis LB, Lessard PA, Ghiviriga I, Samb and an TG, Rha CK, Sinskey AJ.
Journal: Appl Microbiol Biotechnol (2006): 145
Downregulation of inhibitor of apoptosis proteins in apoptotic human chondrocytes treated with tumor necrosis factor-alpha and actinomycin D
Authors: Yoshimura F, Kanno H, Uzuki M, Tajima K, Shimamura T, Sawai T.
Journal: Osteoarthritis Cartilage (2006): 435
Anti-apoptotic effect of hepatocyte growth factor from actinomycin D in hepatocyte-derived HL7702 cells is associated with activation of PI3K/Akt signaling
Authors: Li W, Cai S, Cai L, Li X.
Journal: Toxicol Lett (2006): 142
Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway
Authors: Kim HS, Loughran PA, Kim PK, Billiar TR, Zuckerbraun BS.
Journal: Biochem Biophys Res Commun (2006): 1172
Single-dose actinomycin D: Efficacy in the prophylaxis of postmolar gestational trophoblastic neoplasia in adolescents with high-risk hydatidiform mole
Authors: Uberti EM, Diestel MC, Guimaraes FE, De Napoli G, Schmid H.
Journal: Gynecol Oncol (2006): 325
Prompt non-stacking binding of actinomycin D to hairpin oligonucleotide HP1 and slow redistribution from HP1 to DNA
Authors: Vekshin N, Kovalev A.
Journal: J Biochem (Tokyo) (2006): 185
Evaluation of an actinomycin-D-containing combination chemotherapy protocol with extended maintenance therapy for canine lymphoma
Authors: Siedlecki CT, Kass PH, Jakubiak MJ, Dank G, Lyons J, Kent MS.
Journal: Can Vet J (2006): 52
Dexamethasone, melphalan, actinomycin D, cytosine arabinoside (DMAC) protocol for dogs with relapsed lymphoma
Authors: Alvarez FJ, Kisseberth WC, Gallant SL, Couto CG.
Journal: J Vet Intern Med (2006): 1178
Expression of cell kinetics and death during monocyte-macrophage differentiation: effects of Actinomycin D and Vinblastine treatments
Authors: Spano A, Monaco G, Barni S, Sciola L.
Journal: Histochem Cell Biol. (2006)
Binding of actinomycin C1 (D) and actinomin to base-modified oligonucleotide duplexes with parallel chain orientation
Authors: Li H, Peng X, Leonard P, Seela F.
Journal: Bioorg Med Chem (2006): 4089