Actinomycin D

Actinomycin is a polypeptide antibiotic isolated from soil bacteria of the genus Streptomyces. It binds to DNA duplexes, thereby interfering with the action of enzymes engaged in replication and transcription. Actinomycin D is one of the old chemotherapy drugs which have been used in therapy for many years.

Protocol

SDS

COO

COA

Catalog	Size	Price	Quantity
17505	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actinomycin D to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	79.655 µL	398.273 µL	796.546 µL	3.983 mL	7.965 mL
5 mM	15.931 µL	79.655 µL	159.309 µL	796.546 µL	1.593 mL
10 mM	7.965 µL	39.827 µL	79.655 µL	398.273 µL	796.546 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1255.42 g/mol	=	mL	x	mM	=	mmol

Citations

View all 18 citations: Citation Explorer

circHMGCS1 Promotes the Progression of NSCLC by Regulating the HuR/SFPQ/TNF Pathway

Authors:

An, Jiayue and Sun, Weimiao and Ti, Chenhui and Mu, Yulong and Yin, Baohui and Wang, Qin and Zhang, Xia and Liang, Yan and Sun, Hongfang and Xie, Shuyang and others,

Journal:

The FASEB Journal (2025): e71002

m6A-mediated DGUOK-AS1 stabilized by RBM15 and HNRNPH1 promotes lung adenocarcinoma progression

Authors:

Yang, Menghao and Li, Hui and Zhang, Hongrong and Wu, Jiaen and Li, Youjie and Liang, Yan and Wang, Qin and Wang, Pingyu and Yu, Yuan and Sun, Guangbin and others,

N6-methyladenosine-modified circ\_0000517 promotes non-small cell lung cancer metastasis via miR-1233-3p/CDH6 axis

Authors:

Lin, Weixian and Huang, Lifang and Ou, Zhu’an and Xuan, Yiwen and Zhu, Daoqi and Zhang, Qipeng and Xu, Enwu

Journal:

Journal of Molecular Histology (2025): 1--13

hsa\_circ\_0008305 facilitates the malignant progression of hepatocellular carcinoma by regulating AKR1C3 expression and sponging miR-379-5p

Authors:

Huang, Shenglan and Liu, Kan and Xu, Yongkan and Wang, Hua and Fu, Shumin and Wu, Jianbing

Journal:

Scientific Reports (2025): 1309

Hypoxia-driven M2-polarized macrophages facilitate the epithelial-mesenchymal transition of glioblastoma via extracellular vesicles

Authors:

Liu, Liang and Wang, Ran and Alifu, Aogesi and Xiao, Yong and Liu, Yong and Qian, Chunfa and Zhao, Mengjie and Tang, Xianglong and Xie, Yandong and Shi, Yan and others,

Journal:

Theranostics (2024): 6392

References

View all 66 references: Citation Explorer

A liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of actinomycin-D and vincristine in children with cancer

Authors:

Skolnik JM, Barrett JS, Shi H, Adamson PC.

Journal:

Cancer Chemother Pharmacol (2006): 458

Downregulation of inhibitor of apoptosis proteins in apoptotic human chondrocytes treated with tumor necrosis factor-alpha and actinomycin D

Authors:

Yoshimura F, Kanno H, Uzuki M, Tajima K, Shimamura T, Sawai T.

Journal:

Osteoarthritis Cartilage (2006): 435

Anti-apoptotic effect of hepatocyte growth factor from actinomycin D in hepatocyte-derived HL7702 cells is associated with activation of PI3K/Akt signaling

Authors:

Li W, Cai S, Cai L, Li X.

Journal:

Toxicol Lett (2006): 142

Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway

Authors:

Kim HS, Loughran PA, Kim PK, Billiar TR, Zuckerbraun BS.

Journal:

Biochem Biophys Res Commun (2006): 1172

Single-dose actinomycin D: Efficacy in the prophylaxis of postmolar gestational trophoblastic neoplasia in adolescents with high-risk hydatidiform mole

Authors:

Uberti EM, Diestel MC, Guimaraes FE, De Napoli G, Schmid H.

Journal:

Gynecol Oncol (2006): 325

Physical properties

Molecular weight	1255.42
Solvent	DMSO

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	50-76-0

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Page updated on October 13, 2025