Name: ADP-TAMRA conjugate [5-TAMRA-eda-ADP]
Brand: AAT Bioquest
SKU: 13606
Price: 582 USD
Availability: InStock

ADP-TAMRA conjugate

5-TAMRA-eda-ADP

Fluorescently labeled ADP molecules are used to screening ADP-binding enzymes and other protein targets for drug discovery. This ADP-TAMRA has been tested for binding kynurenine monooxygenase (KMO) with a K(d) value of 0.60 ± 0.05 ?M and to the NMOs from Aspergillus fumigatus and Mycobacterium smegmatis with K(d) values of 2.1 ± 0.2 and 4.0 ± 0.2 ?M, respectively (Anal Biochem. 2012, 425, 80-7). The assay was tested in competitive binding experiments with substrates and products of KMO and an NMO. NMOs are essential for pathogenesis in fungi and bacteria. NMOs catalyze the hydroxylation of sine and ornithine in the biosynthesis of hydroxamate-containing siderophores. Inhibition of KMO, which catalyzes the conversion of kynurenine to 3-hydroxykynurenine, alleviates neurodegenerative disorders such as Huntington's and Alzheimer's diseases and brain infections caused by the parasite Trypanosoma brucei.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13606	100 nmol	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ADP-TAMRA conjugate [5-TAMRA-eda-ADP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	76.377 µL	381.883 µL	763.767 µL	3.819 mL	7.638 mL
5 mM	15.275 µL	76.377 µL	152.753 µL	763.767 µL	1.528 mL
10 mM	7.638 µL	38.188 µL	76.377 µL	381.883 µL	763.767 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1309.3 g/mol	=	mL	x	mM	=	mmol

References

View all 32 references: Citation Explorer

Deciphering the catalysis-associated conformational changes of human adenylate kinase 1 with single-molecule spectroscopy

Authors:

Lin CY, Huang JY, Lo LW.

Journal:

J Phys Chem B (2013): 13947

The Escherichia coli PriA helicase-double-stranded DNA complex: location of the strong DNA-binding subsite on the helicase domain of the protein and the affinity control by the two nucleotide-binding sites of the enzyme

Authors:

Szymanski MR, Jezewska MJ, Bujalowski W.

Journal:

J Mol Biol (2010): 344

The effect of NBD-Cl in nucleotide-binding of the major subunit alpha and B of the motor proteins F1FO ATP synthase and A1AO ATP synthase

Authors:

Hunke C, Tadwal VS, Manimekalai MS, Roessle M, Gruber G.

Journal:

J Bioenerg Biomembr (2010): 1

ATP/ADP binding to a novel nucleotide binding domain of the reticulocyte-binding protein Py235 of Plasmodium yoelii

Authors:

Ramalingam JK, Hunke C, Gao X, Gruber G, Preiser PR.

Journal:

J Biol Chem (2008): 36386

Reversal of ADP-mediated aggregation of adenosine kinase by cyclophilin leads to its reactivation

Authors:

Sen B, Chakraborty A, Datta R, Bhattacharyya D, Datta AK.

Journal:

Biochemistry (2006): 263

Physical properties

Molecular weight	1309.30
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	552
Emission (nm)	578

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025