Name: AFC [7-Amino-4-trifluoromethylcoumarin] *CAS 53518-15-3*
Brand: AAT Bioquest
SKU: 56
Availability: InStock

AFC

7-Amino-4-trifluoromethylcoumarin; CAS 53518-15-3

This compound is the precursor for a variety of AFC enzyme substrates. It is also used as a calibration standard for AFC-based enzyme substrates. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC.

Protocol

COA

Catalog	Size	Price
56	1 g	Price
57	5 g	Price
58	25 g	Price

References

View all 27 references: Citation Explorer

A study of quenching effect of sulfur-containing amino acids L-cysteine and L-methionine on peroxyoxalate chemiluminescence of 7-amino-4-trifluoromethylcumarin

Authors:

Shamsipur M, Chaichi MJ.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2005): 1227

Ultrafast dynamics of the azobenzene-coumarin complex: investigation of cooling dynamics measured by an integrated molecular thermometer

Authors:

Velate S, Danilov EO, Rodgers MA.

Journal:

J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 8969

Chemiluminescence characteristics of cumarin derivatives as blue fluorescers in peroxyoxalate-hydrogen peroxide system

Authors:

Chaichi MJ, Karami AR, Shockravi A, Shamsipur M.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2003): 1145

Drug resistance towards etoposide and cisplatin in human melanoma cells is associated with drug-dependent apoptosis deficiency

Authors:

Helmbach H, Kern MA, Rossmann E, Renz K, Kissel C, Gschwendt B, Schadendorf D.

Journal:

J Invest Dermatol (2002): 923

Characterization of a cytosolic aminopeptidase from Encephalitozoon intestinalis

Authors:

Millership JJ, Chappell CL, Okhuysen PC, Snowden KF.

Journal:

Parasitology (2002): 1

Physical properties

Molecular weight	229.15
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000 ¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	53518-15-3

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Page updated on October 13, 2025