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AFC [7-Amino-4-trifluoromethylcoumarin] *CAS 53518-15-3*

This compound is the precursor for a variety of AFC enzyme substrates. It is also used as a calibration standard for AFC-based enzyme substrates. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC.

Spectrum

References

View all 27 references: Citation Explorer
A study of quenching effect of sulfur-containing amino acids L-cysteine and L-methionine on peroxyoxalate chemiluminescence of 7-amino-4-trifluoromethylcumarin
Authors: Shamsipur M, Chaichi MJ.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1227
Ultrafast dynamics of the azobenzene-coumarin complex: investigation of cooling dynamics measured by an integrated molecular thermometer
Authors: Velate S, Danilov EO, Rodgers MA.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 8969
Chemiluminescence characteristics of cumarin derivatives as blue fluorescers in peroxyoxalate-hydrogen peroxide system
Authors: Chaichi MJ, Karami AR, Shockravi A, Shamsipur M.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2003): 1145
Drug resistance towards etoposide and cisplatin in human melanoma cells is associated with drug-dependent apoptosis deficiency
Authors: Helmbach H, Kern MA, Rossmann E, Renz K, Kissel C, Gschwendt B, Schadendorf D.
Journal: J Invest Dermatol (2002): 923
Characterization of a cytosolic aminopeptidase from Encephalitozoon intestinalis
Authors: Millership JJ, Chappell CL, Okhuysen PC, Snowden KF.
Journal: Parasitology (2002): 1
Page updated on December 6, 2024

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Catalog Number
565758
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Physical properties

Molecular weight

229.15

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

170001

Excitation (nm)

376

Emission (nm)

482

Quantum yield

0.531

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

53518-15-3
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