AFC [7-Amino-4-trifluoromethylcoumarin] CAS 53518-15-3

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	229.15
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

53518-15-3

Molecular weight

229.15

Extinction coefficient (cm ^-1 M ^-1)

17000¹

Excitation (nm)

376

Emission (nm)

482

Quantum yield

0.53¹

This compound is the precursor for a variety of AFC enzyme substrates. It is also used as a calibration standard for AFC-based enzyme substrates. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AFC [7-Amino-4-trifluoromethylcoumarin] *CAS 53518-15-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	436.395 µL	2.182 mL	4.364 mL	21.82 mL	43.64 mL
5 mM	87.279 µL	436.395 µL	872.791 µL	4.364 mL	8.728 mL
10 mM	43.64 µL	218.198 µL	436.395 µL	2.182 mL	4.364 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53¹

Images

Figure 1. Chemical structure for AFC [7-Amino-4-trifluoromethylcoumarin] *CAS 53518-15-3*

References

View all 27 references: Citation Explorer

A study of quenching effect of sulfur-containing amino acids L-cysteine and L-methionine on peroxyoxalate chemiluminescence of 7-amino-4-trifluoromethylcumarin
Authors: Shamsipur M, Chaichi MJ.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1227

Ultrafast dynamics of the azobenzene-coumarin complex: investigation of cooling dynamics measured by an integrated molecular thermometer
Authors: Velate S, Danilov EO, Rodgers MA.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 8969

Chemiluminescence characteristics of cumarin derivatives as blue fluorescers in peroxyoxalate-hydrogen peroxide system
Authors: Chaichi MJ, Karami AR, Shockravi A, Shamsipur M.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2003): 1145

Drug resistance towards etoposide and cisplatin in human melanoma cells is associated with drug-dependent apoptosis deficiency
Authors: Helmbach H, Kern MA, Rossmann E, Renz K, Kissel C, Gschwendt B, Schadendorf D.
Journal: J Invest Dermatol (2002): 923

Characterization of a cytosolic aminopeptidase from Encephalitozoon intestinalis
Authors: Millership JJ, Chappell CL, Okhuysen PC, Snowden KF.
Journal: Parasitology (2002): 1

A fluorescent anion sensor that works in neutral aqueous solution for bioanalytical application
Authors: Mizukami S, Nagano T, Urano Y, Odani A, Kikuchi K.
Journal: J Am Chem Soc (2002): 3920

Quenching effect of triethylamine on peroxyoxalate chemiluminescence in the presence of 7-amino-4-trifluoromethylcumarin
Authors: Shamsipur M, Chaichi MJ.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2001): 2355

The appearance and possible role of plasminogen activator of urokinase type (u-PA) activity in the cornea related to soft contact lens wear in rabbits
Authors: Cejkova J., undefined
Journal: Doc Ophthalmol (1998): 165

Histochemical study of leukocyte elastase activity in alkali-burned rabbit cornea
Authors: Cejkova J., undefined
Journal: Ophthalmic Res (1997): 154

Bacterial proteases in gingival crevicular fluid before and after periodontal treatment
Authors: Eley BM, Cox SW.
Journal: Br Dent J (1995): 133