Name: Ala-AMC
Brand: AAT Bioquest
SKU: 13458
Price: 115 USD
Availability: InStock

Ala-AMC

Ala-AMC is a sensitive fluorogenic protease substrate. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of alanyl aminopeptidase and trypsin.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13458	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ala-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	277.562 µL	1.388 mL	2.776 mL	13.878 mL	27.756 mL
5 mM	55.512 µL	277.562 µL	555.124 µL	2.776 mL	5.551 mL
10 mM	27.756 µL	138.781 µL	277.562 µL	1.388 mL	2.776 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	360.28 g/mol	=	mL	x	mM	=	mmol

References

View all 79 references: Citation Explorer

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease

Authors:

Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.

Journal:

Anal Biochem (2009): 195

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model

Authors:

Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.

Journal:

Gynecol Oncol (2009): 616

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7

Authors:

Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.

Journal:

J Biomol Screen (2009): 1

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

Authors:

Nakata H, Ohtsuki T, Sisido M.

Journal:

Anal Biochem (2009): 121

Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes

Authors:

An L, Liu L, Wang S.

Journal:

Biomacromolecules (2009): 454

Physical properties

Molecular weight	360.28
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	96594-10-4

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Page updated on October 12, 2025