Alkaline Phosphatase [CIAP]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~86 kDa |
Solvent | Water |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Alkaline Phosphatase [CIAP] *Stabilized in aqueous solution* |
Overview | SDSProtocol |
See also: Alkaline Phosphatase (ALP)
Molecular weight ~86 kDa |
Calf Intestinal Alkaline Phosphatase (CIP or CIAP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. CIAP also hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). CIAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5´ protruding, 5´ recessed and blunt ends. CIAP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. CIAP conjugates have been widely used in immunological assays and tests in combination with a variety of color or fluorogenic AP substrates. AAT Bioquest offers the largest collection of fluorogenic AP substrates for developing AP-based ELISA assays and tests.
Images
Figure 1. Calf Intestinal Alkaline Phosphatase (CIP or CIAP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. CIAP also hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). CIAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. CIAP conjugates have been widely used in immunological assays and tests in combination with a variety of color or fluorogenic AP substrates. AAT Bioquest offers the largest collection of fluorogenic AP substrates for developing AP-based ELISA assays and tests.
References
View all 20 references: Citation Explorer
Effects of intestinal alkaline phosphatase on intestinal barrier function in a cecal ligation and puncture (CLP)-induced mouse model for sepsis.
Authors: Plaeke, Philip and De Man, Joris G and Smet, Annemieke and Malhotra-Kumar, Surbhi and Pintelon, Isabel and Timmermans, Jean-Pierre and Nullens, Sara and Jorens, Philippe G and Hubens, Guy and De Winter, Benedicte Y
Journal: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2020): e13754
Authors: Plaeke, Philip and De Man, Joris G and Smet, Annemieke and Malhotra-Kumar, Surbhi and Pintelon, Isabel and Timmermans, Jean-Pierre and Nullens, Sara and Jorens, Philippe G and Hubens, Guy and De Winter, Benedicte Y
Journal: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2020): e13754
CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.
Authors: Verheijen, Nanne and Suttorp, Christiaan M and van Rheden, René E M and Regan, Raymond F and Helmich, Maria P A C and Kuijpers-Jagtman, Anne Marie and Wagener, Frank A D T G
Journal: Frontiers in cell and developmental biology (2020): 771
Authors: Verheijen, Nanne and Suttorp, Christiaan M and van Rheden, René E M and Regan, Raymond F and Helmich, Maria P A C and Kuijpers-Jagtman, Anne Marie and Wagener, Frank A D T G
Journal: Frontiers in cell and developmental biology (2020): 771
Loss of tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity in cerebral microvessels is coupled to persistent neuroinflammation and behavioral deficits in late sepsis.
Authors: Nwafor, Divine C and Chakraborty, Sreeparna and Brichacek, Allison L and Jun, Sujung and Gambill, Catheryne A and Wang, Wei and Engler-Chiurazzi, Elizabeth B and Dakhlallah, Duaa and Pinkerton, Anthony B and Millán, José Luis and Benkovic, Stanley A and Brown, Candice M
Journal: Brain, behavior, and immunity (2020): 115-131
Authors: Nwafor, Divine C and Chakraborty, Sreeparna and Brichacek, Allison L and Jun, Sujung and Gambill, Catheryne A and Wang, Wei and Engler-Chiurazzi, Elizabeth B and Dakhlallah, Duaa and Pinkerton, Anthony B and Millán, José Luis and Benkovic, Stanley A and Brown, Candice M
Journal: Brain, behavior, and immunity (2020): 115-131
Protective role of thymoquinone in sepsis-induced liver injury in BALB/c mice.
Authors: Wang, Fei and Lei, Xiong and Zhao, Yue and Yu, Qinggong and Li, Qianwei and Zhao, Hui and Pei, Zuowei
Journal: Experimental and therapeutic medicine (2019): 1985-1992
Authors: Wang, Fei and Lei, Xiong and Zhao, Yue and Yu, Qinggong and Li, Qianwei and Zhao, Hui and Pei, Zuowei
Journal: Experimental and therapeutic medicine (2019): 1985-1992
Considering the Effect of Rosa damascena Mill. Essential Oil on Oxidative Stress and COX-2 Gene Expression in the Liver of Septic Rats.
Authors: Dadkhah, Abolfazl and Fatemi, Faezeh and Malayeri, Mohammad Reza Mohammadi and Ashtiyani, Mohammad Hassan Karvin and Noureini, Sakineh Kazemi and Rasooli, Azadeh
Journal: Turkish journal of pharmaceutical sciences (2019): 416-424
Authors: Dadkhah, Abolfazl and Fatemi, Faezeh and Malayeri, Mohammad Reza Mohammadi and Ashtiyani, Mohammad Hassan Karvin and Noureini, Sakineh Kazemi and Rasooli, Azadeh
Journal: Turkish journal of pharmaceutical sciences (2019): 416-424
Multiple layers of regulation determine the cellular levels of the Pup ligase PafA in Mycobacterium smegmatis.
Authors: Korman, Maayan and Schlussel, Shai and Vishkautzan, Marina and Gur, Eyal
Journal: Molecular microbiology (2019): 620-631
Authors: Korman, Maayan and Schlussel, Shai and Vishkautzan, Marina and Gur, Eyal
Journal: Molecular microbiology (2019): 620-631
Hepatoprotective Effect of Echinochrome Pigment in Septic Rats.
Authors: Mohamed, Ayman S and Sadek, Shimaa A and Hassanein, Sarah S and Soliman, Amel M
Journal: The Journal of surgical research (2019): 317-324
Authors: Mohamed, Ayman S and Sadek, Shimaa A and Hassanein, Sarah S and Soliman, Amel M
Journal: The Journal of surgical research (2019): 317-324
Overexpression of homeodomain-interacting protein kinase 2 (HIPK2) attenuates sepsis-mediated liver injury by restoring autophagy.
Authors: Jiang, Zhengyu and Bo, Lulong and Meng, Yan and Wang, Chen and Chen, Tianxing and Wang, Changli and Yu, Xiya and Deng, Xiaoming
Journal: Cell death & disease (2018): 847
Authors: Jiang, Zhengyu and Bo, Lulong and Meng, Yan and Wang, Chen and Chen, Tianxing and Wang, Changli and Yu, Xiya and Deng, Xiaoming
Journal: Cell death & disease (2018): 847
Hepatoprotective Effect of Essential Oils from Hyptis crenata in Sepsis-Induced Liver Dysfunction.
Authors: Lima, Glauber Cruz and Vasconcelos, Yuri de Abreu Gomes and de Santana Souza, Marilia Trindade and Oliveira, Alan Santos and Bomfim, Rangel Rodrigues and de Albuquerque Júnior, Ricardo Luiz Cavalcanti and Camargo, Enilton Aparecido and Portella, Viviane Gomes and Coelho-de-Souza, Andrelina Noronha and Diniz, Lúcio Ricardo Leite
Journal: Journal of medicinal food (2018): 709-715
Authors: Lima, Glauber Cruz and Vasconcelos, Yuri de Abreu Gomes and de Santana Souza, Marilia Trindade and Oliveira, Alan Santos and Bomfim, Rangel Rodrigues and de Albuquerque Júnior, Ricardo Luiz Cavalcanti and Camargo, Enilton Aparecido and Portella, Viviane Gomes and Coelho-de-Souza, Andrelina Noronha and Diniz, Lúcio Ricardo Leite
Journal: Journal of medicinal food (2018): 709-715
Insulin Modulates Liver Function in a Type I Diabetes Rat Model.
Authors: Nolasco, Eduardo L and Zanoni, Fernando L and Nunes, Fernanda P B and Ferreira, Sabrina S and Freitas, Luiza A and Silva, Mariana C F and Martins, Joilson O
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2015): 1467-79
Authors: Nolasco, Eduardo L and Zanoni, Fernando L and Nunes, Fernanda P B and Ferreira, Sabrina S and Freitas, Luiza A and Silva, Mariana C F and Martins, Joilson O
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2015): 1467-79
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Biotin Labeling Molecules and Their Biological Applications
FAQ
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
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Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?