Alkaline Phosphatase [CIAP] *Stabilized in aqueous solution*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~86 kDa |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
Alkaline Phosphatase [CIAP] |
Overview | SDSProtocol |
See also: Alkaline Phosphatase (ALP)
Molecular weight ~86 kDa |
Calf Intestinal Alkaline Phosphatase (CIP or CIAP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. CIAP also hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). CIAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5´ protruding, 5´ recessed and blunt ends. CIAP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. CIAP conjugates have been widely used in immunological assays and tests in combination with a variety of color or fluorogenic AP substrates. AAT Bioquest offers the largest collection of fluorogenic AP substrates for developing AP-based ELISA assays and tests.
Images
Figure 1. Calf Intestinal Alkaline Phosphatase (CIP or CIAP) nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. CIAP also hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). CIAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. CIAP conjugates have been widely used in immunological assays and tests in combination with a variety of color or fluorogenic AP substrates. AAT Bioquest offers the largest collection of fluorogenic AP substrates for developing AP-based ELISA assays and tests.
Application notes
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FAQ
What are the major steps involved in RNA processing?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?