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AMCA Alkyne

AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling alkyne compounds through click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM369.987 µL1.85 mL3.7 mL18.499 mL36.999 mL
5 mM73.997 µL369.987 µL739.973 µL3.7 mL7.4 mL
10 mM36.999 µL184.993 µL369.987 µL1.85 mL3.7 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA acid *CAS#: 106562-32-7*346434190000.153
AMCA Ethylenediamine346434190000.153
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*499520710000.11
ICG alkyne7898132300000.076
Cy3B alkyne56057112000010.069
XFD647 Alkyne6506712700000.03
XFD350 alkyne343441190000.19
XFD405 alkyne40142135,0000.70
XFD430 alkyne43254015,0000.28
XFD514 alkyne518543800000.18
XFD546 alkyne5615721120000.12
XFD532 alkyne534553810000.09
XFD568 alkyne579603913000.46
XFD594 alkyne590618900000.56
XFD610 alkyne611629144,0000.44
Show More (6)

References

View all 13 references: Citation Explorer
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
Page updated on December 11, 2024

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Physical properties

Molecular weight

270.28

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.153

Extinction coefficient (cm -1 M -1)

19000

Excitation (nm)

346

Emission (nm)

434

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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