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AMCA Alkyne

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Physical properties
Molecular weight270.28
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.153
Extinction coefficient (cm -1 M -1)19000
Excitation (nm)346
Emission (nm)434
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Coumarins
Molecular weight
270.28
Correction Factor (280 nm)
0.153
Extinction coefficient (cm -1 M -1)
19000
Excitation (nm)
346
Emission (nm)
434
AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling alkyne compounds through click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM369.987 µL1.85 mL3.7 mL18.499 mL36.999 mL
5 mM73.997 µL369.987 µL739.973 µL3.7 mL7.4 mL
10 mM36.999 µL184.993 µL369.987 µL1.85 mL3.7 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.153
Extinction coefficient (cm -1 M -1)19000
Excitation (nm)346
Emission (nm)434

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA acid *CAS#: 106562-32-7*346434190000.153
AMCA Ethylenediamine346434190000.153
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*499520710000.11
ICG alkyne7898132300000.076
Cy3B alkyne56057112000010.069
XFD647 Alkyne6506712700000.03

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References


View all 13 references: Citation Explorer
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