AMCA Azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	301.30
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.153
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	346
Emission (nm)	434

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

301.30

Correction Factor (280 nm)

0.153

Extinction coefficient (cm ^-1 M ^-1)

19000

Excitation (nm)

346

Emission (nm)

434

AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling azide compounds through click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	331.895 µL	1.659 mL	3.319 mL	16.595 mL	33.19 mL
5 mM	66.379 µL	331.895 µL	663.79 µL	3.319 mL	6.638 mL
10 mM	33.19 µL	165.948 µL	331.895 µL	1.659 mL	3.319 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Correction Factor (280 nm)	0.153
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	346
Emission (nm)	434

Product Family

Name	Excitation (nm)	Emission (nm)	Correction Factor (280 nm)
AMCA acid CAS#: 106562-32-7	346	434	0.153
AMCA Ethylenediamine	346	434	0.153
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	499	520	0.11
ICG azide	789	814	0.076
Cy3B azide	560	571	0.069
XFD647 Azide	650	671	0.03

Images

Figure 1. Chemical structure for AMCA Azide

References

View all 13 references: Citation Explorer

Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1

Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
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Journal: Anal Biochem (2003): 160

Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
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Journal: Int J Oral Maxillofac Surg (2001): 518

Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
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Journal: Mund Kiefer Gesichtschir (2000): 240

Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681

Soluble aggregates of IgG and immune complexes enhance IL-6 production by renal mesangial cells
Authors: van den Dobbelsteen ME, van der Woude FJ, Schroeijers WE, van Es LA, Daha MR.
Journal: Kidney Int (1993): 544

Evidence for cobinding of self- and allopeptides to human class II major histocompatibility antigen DR1 by energy transfer
Authors: Kropshofer H, Max H, Kalbacher H.
Journal: Proc Natl Acad Sci U S A (1993): 403

Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide
Authors: Eis PS, Lakowicz JR.
Journal: Biochemistry (1993): 7981

Improved indirect fluorescence immunocytochemical method using counterstains
Authors: Newkirk RF, Mack J.
Journal: Biotechniques (1992): 536

Triple immunofluorescence confocal laser scanning microscopy: spatial correlation of novel cellular differentiation markers in human muscle biopsies
Authors: Schubert W., undefined
Journal: Eur J Cell Biol (1991): 272