AMCA Ethylenediamine

AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling carbonyl compounds (such as carboxy compounds).

Protocol

SDS

Catalog	Size	Price	Quantity
504	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	198.669 µL	993.345 µL	1.987 mL	9.933 mL	19.867 mL
5 mM	39.734 µL	198.669 µL	397.338 µL	1.987 mL	3.973 mL
10 mM	19.867 µL	99.334 µL	198.669 µL	993.345 µL	1.987 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	503.35 g/mol	=	mL	x	mM	=	mmol

References

View all 13 references: Citation Explorer

Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system

Authors:

Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.

Journal:

J Comp Neurol (2005): 1

Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles

Authors:

Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.

Journal:

Anal Biochem (2003): 160

Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding

Authors:

Blinder D, Manor Y, Martinowitz U, Taicher S.

Journal:

Int J Oral Maxillofac Surg (2001): 518

Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid

Authors:

Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.

Journal:

Mund Kiefer Gesichtschir (2000): 240

Oral surgical procedures during anticoagulant therapy

Authors:

Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.

Journal:

Harefuah (1996): 681

Physical properties

Molecular weight	503.35
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.153
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	346
Emission (nm)	434

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Oligonucleotide Labeling Reagents
Brochure: Labeling Antibodies & Biopolymers
Brochure: Peptide Labeling Reagents

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Page updated on October 30, 2025