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AMCA, succinimidyl ester

AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. AMCA, succinimidyl ester can be used for labeling aliphatic amine compounds.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM302.764 µL1.514 mL3.028 mL15.138 mL30.276 mL
5 mM60.553 µL302.764 µL605.528 µL3.028 mL6.055 mL
10 mM30.276 µL151.382 µL302.764 µL1.514 mL3.028 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 13 references: Citation Explorer
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
Page updated on September 19, 2024

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Physical properties

Molecular weight

330.29

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.153

Extinction coefficient (cm -1 M -1)

19000

Excitation (nm)

346

Emission (nm)

434

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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