Name: AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9*
Brand: AAT Bioquest
SKU: 206
Price: 115 USD
Availability: InStock

AMF

4'-(Aminomethyl)fluorescein; CAS 91539-64-9

4'-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
206	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	216.193 µL	1.081 mL	2.162 mL	10.81 mL	21.619 mL
5 mM	43.239 µL	216.193 µL	432.386 µL	2.162 mL	4.324 mL
10 mM	21.619 µL	108.096 µL	216.193 µL	1.081 mL	2.162 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	462.55 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria

Authors:

Chen, Yuwan and Zhou, Wen and Xia, Yufei and Zhang, Weijie and Zhao, Qun and Li, Xinwei and Gao, Hang and Liang, Zhen and Ma, Guanghui and Yang, Kaiguang and others,

Journal:

Nature Communications (2023): 3882

Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model

Authors:

Wang, Yulong and Li, Zhenfeng and Barnych, Bogdan and Huo, Jingqian and Wan, Debin and Vasylieva, Natalia and Xu, Junli and Li, Pan and Liu, Beibei and Zhang, Cunzheng and others,

Journal:

Journal of agricultural and food chemistry (2019): 11536--11541

References

View all 15 references: Citation Explorer

Type and location of fluorescent probes incorporated into the potent mu-opioid peptide [Dmt]DALDA affect potency, receptor selectivity and intrinsic efficacy

Authors:

Schiller PW, Berezowska I, Weltrowska G, Chen H, Lemieux C, Chung NN.

Journal:

J Pept Res (2005): 556

Assessment of S-nitrosothiols on diaminofluorescein gels

Authors:

King M, Gildemeister O, Gaston B, Mannick JB.

Journal:

Anal Biochem (2005): 69

Detection of polychlorinated biphenyls using an antibody column in tandem with a fluorescent liposome column. Effect of albumin on phospholipase A2-catalyzed membrane leakage

Authors:

Liu XY, Nakamura C, Nakamura N, Hirano T, Shinbo T, Miyake J.

Journal:

J Chromatogr A (2005): 229

Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery

Authors:

Dubruel P, Christiaens B, Rosseneu M, V and ekerckhove J, Grooten J, Goossens V, Schacht E.

Journal:

Biomacromolecules (2004): 379

Effect of glyphosate on the microbial activity of two Brazilian soils

Authors:

Araujo AS, Monteiro RT, Abarkeli RB.

Journal:

Chemosphere (2003): 799

Physical properties

Molecular weight	462.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	91539-64-9

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Page updated on October 13, 2025