AMF [4'-(Aminomethyl)fluorescein] CAS 91539-64-9

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Physical properties

Molecular weight	462.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	216.193 µL	1.081 mL	2.162 mL	10.81 mL	21.619 mL
5 mM	43.239 µL	216.193 µL	432.386 µL	2.162 mL	4.324 mL
10 mM	21.619 µL	108.096 µL	216.193 µL	1.081 mL	2.162 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Chemical structure for AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9*

Citations

View all 2 citations: Citation Explorer

Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria
Authors: Chen, Yuwan and Zhou, Wen and Xia, Yufei and Zhang, Weijie and Zhao, Qun and Li, Xinwei and Gao, Hang and Liang, Zhen and Ma, Guanghui and Yang, Kaiguang and others,
Journal: Nature Communications (2023): 3882

Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model
Authors: Wang, Yulong and Li, Zhenfeng and Barnych, Bogdan and Huo, Jingqian and Wan, Debin and Vasylieva, Natalia and Xu, Junli and Li, Pan and Liu, Beibei and Zhang, Cunzheng and others,
Journal: Journal of agricultural and food chemistry (2019): 11536--11541

References

View all 15 references: Citation Explorer

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Journal: Anal Biochem (2005): 69

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Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery
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Amyloid beta-protein affects cholesterol metabolism in cultured neurons: implications for pivotal role of cholesterol in the amyloid cascade
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Antisense knockdown of inducible nitric oxide synthase inhibits the relaxant effect of VIP in isolated smooth muscle cells of the mouse gastric fundus
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Journal: Br J Pharmacol (2001): 425

A capillary electrophoresis laser-induced fluorescence method for analysis of potato glycoalkaloids based on a solution-phase immunoassay. 1. Separation and quantification of immunoassay products
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Journal: J Agric Food Chem (2000): 1135

Relationship between cytotoxic drug response patterns and activity of drug efflux transporters mediating multidrug resistance
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Adaptive bone remodeling around oral implants under axial and nonaxial loading conditions in the dog mandible
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Journal: Int J Oral Maxillofac Implants (1997): 215