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AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9*

4'-(Aminomethyl)fluorescein is an excellent building block for developing fluoresceinated bioconjugates. Its amino group readily reacts with activated carboxy esters (such as NHS ester).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AMF [4'-(Aminomethyl)fluorescein] *CAS 91539-64-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM216.193 µL1.081 mL2.162 mL10.81 mL21.619 mL
5 mM43.239 µL216.193 µL432.386 µL2.162 mL4.324 mL
10 mM21.619 µL108.096 µL216.193 µL1.081 mL2.162 mL

Molarity calculator

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Spectrum

Citations

View all 2 citations: Citation Explorer
Targeted cross-linker delivery for the in situ mapping of protein conformations and interactions in mitochondria
Authors: Chen, Yuwan and Zhou, Wen and Xia, Yufei and Zhang, Weijie and Zhao, Qun and Li, Xinwei and Gao, Hang and Liang, Zhen and Ma, Guanghui and Yang, Kaiguang and others,
Journal: Nature Communications (2023): 3882
Investigation of the Small Size of Nanobodies for a Sensitive Fluorescence Polarization Immunoassay for Small Molecules: 3-Phenoxybenzoic Acid, an Exposure Biomarker of Pyrethroid Insecticides as a Model
Authors: Wang, Yulong and Li, Zhenfeng and Barnych, Bogdan and Huo, Jingqian and Wan, Debin and Vasylieva, Natalia and Xu, Junli and Li, Pan and Liu, Beibei and Zhang, Cunzheng and others,
Journal: Journal of agricultural and food chemistry (2019): 11536--11541

References

View all 15 references: Citation Explorer
Type and location of fluorescent probes incorporated into the potent mu-opioid peptide [Dmt]DALDA affect potency, receptor selectivity and intrinsic efficacy
Authors: Schiller PW, Berezowska I, Weltrowska G, Chen H, Lemieux C, Chung NN.
Journal: J Pept Res (2005): 556
Assessment of S-nitrosothiols on diaminofluorescein gels
Authors: King M, Gildemeister O, Gaston B, Mannick JB.
Journal: Anal Biochem (2005): 69
Detection of polychlorinated biphenyls using an antibody column in tandem with a fluorescent liposome column. Effect of albumin on phospholipase A2-catalyzed membrane leakage
Authors: Liu XY, Nakamura C, Nakamura N, Hirano T, Shinbo T, Miyake J.
Journal: J Chromatogr A (2005): 229
Buffering properties of cationic polymethacrylates are not the only key to successful gene delivery
Authors: Dubruel P, Christiaens B, Rosseneu M, V and ekerckhove J, Grooten J, Goossens V, Schacht E.
Journal: Biomacromolecules (2004): 379
Effect of glyphosate on the microbial activity of two Brazilian soils
Authors: Araujo AS, Monteiro RT, Abarkeli RB.
Journal: Chemosphere (2003): 799
Page updated on October 12, 2024

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Physical properties

Molecular weight

462.55

Solvent

DMSO

Spectral properties

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm -1 M -1)

800001

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.79001, 0.952

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

91539-64-9
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