Aminopropargyl dUTP [5-Propargylamino-2'-deoxyuridine-5'-triphosphate]

Aminopropargyl dUTP

5-Propargylamino-2'-deoxyuridine-5'-triphosphate

The amine-modified deoxyuridine 5'-triphosphates (such as 5-propargylamino-2'-deoxyuridine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17053	10 umoles	Price

References

View all 3 references: Citation Explorer

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Fluorescent labelling of cRNA for microarray applications

Authors:

t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.

Journal:

Nucleic Acids Res (2003): e20

Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA

Authors:

Chen CB, Gorin MB, Sigman DS.

Journal:

Proc Natl Acad Sci U S A (1993): 4206

Physical properties

Molecular weight	521.20
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	1380329-75-8

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Page updated on October 12, 2025