logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Amplite® Cholesterol Quantitation Kit
Cholesterol is required to build and maintain cell membranes. It modulates membrane fluidity over the range of physiological temperatures. Within cells, cholesterol is the precursor molecule in several biochemical pathways. Cholesterol is also an important precursor molecule for the synthesis of Vitamin D and the steroid hormones, including the adrenal gland hormones cortisol and aldosterone as well as the sex hormones progesterone, estrogens, together with testosterone and their derivatives. This Amplite® Cholesterol Quantitation Assay Kit provides one of the most sensitive methods for quantifying cholesterol. The kit uses Amplite® Red to quantify the concentration of cholesterol, which is related to the production of hydrogen peroxide in the cholesterol oxidase-mediated enzyme coupling reactions in the presence of cholesterol. The amount of cholesterol is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite® Red is proportional to the concentration of hydrogen peroxide that is converted to the concentration of cholesterol. The assay can be readily read with a fluorescence microplate reader.
Cholesterol dose response was measured with Amplite® Fluorimetric Cholesterol Quantitation Kit in a black 96-well plate using a Gemini fluorescence microplate reader (molecular devices). As low as 0.03 µM cholesterol can be detected with 30 minutes incubation (n=3).
Cholesterol dose response was measured with Amplite® Fluorimetric Cholesterol Quantitation Kit in a black 96-well plate using a Gemini fluorescence microplate reader (molecular devices). As low as 0.03 µM cholesterol can be detected with 30 minutes incubation (n=3).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
40006200 Tests
Price
 
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on September 25, 2025