Amplite® Cholesterol Quantitation Kit

Amplite® Cholesterol Quantitation Kit is a fluorimetric assay kit for quantifying cholesterol through an enzyme-coupled hydrogen peroxide readout in a microplate format.

Fluorimetric detection: Uses Amplite® Red to measure hydrogen peroxide generated by cholesterol oxidase-mediated reactions.
High sensitivity: Detects cholesterol concentrations as low as 0.03 µM after 30 minutes.
Enzyme-coupled chemistry: Converts cholesterol into a proportional hydrogen peroxide signal.
Microplate compatible: Read at Ex/Em = 540/590 nm using a fluorescence microplate reader and solid black plate.
Applications: Suitable for quantitative cholesterol measurements in biochemical and cell metabolism studies.

Cholesterol dose response was measured with Amplite® Fluorimetric Cholesterol Quantitation Kit in a black 96-well plate using a Gemini fluorescence microplate reader (molecular devices). As low as 0.03 µM cholesterol can be detected with 30 minutes incubation (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
40006	200 Tests	Price

Overview

Cholesterol is required to build and maintain cell membranes. It modulates membrane fluidity over the range of physiological temperatures. Within cells, cholesterol is the precursor molecule in several biochemical pathways. Cholesterol is also an important precursor molecule for the synthesis of Vitamin D and the steroid hormones, including the adrenal gland hormones cortisol and aldosterone as well as the sex hormones progesterone, estrogens, together with testosterone and their derivatives. This Amplite® Cholesterol Quantitation Assay Kit provides one of the most sensitive methods for quantifying cholesterol. The kit uses Amplite® Red to quantify the concentration of cholesterol, which is related to the production of hydrogen peroxide in the cholesterol oxidase-mediated enzyme coupling reactions in the presence of cholesterol. The amount of cholesterol is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite® Red is proportional to the concentration of hydrogen peroxide that is converted to the concentration of cholesterol. The assay can be readily read with a fluorescence microplate reader.

Citations

View all 18 citations: Citation Explorer

Lappaconitine hydrobromide prevents NLRP3-GSDMD-mediated pyroptosis to alleviate sepsis-induced acute lung injury by enhancing NR1H3

Authors:

Lan, Yuejia and Zhong, Qingrui and Huang, Hongkai and Deng, Xiao and Yang, Lu and Sun, Jiayi and Liu, Mingyang and Meng, Xianli and Wu, Jiasi

Journal:

Journal of Pharmaceutical Analysis (2026): 101631

25HC Depleted PMs-Accessible Cholesterol to Restrict SFTSV Entry and Infectious-EVs Mediated Infection

Authors:

Zhang, Rui and Li, Linrun and Li, Siyu and Yin, Shengxia and Cheng, Min and Jing, Na and Li, Jie and Wu, Zhiwei and Wu, Chao

Journal:

The FASEB Journal (2025): e70839

Cholesterol depletion suppresses thermal necrosis resistance by alleviating an increase in membrane fluidity

Authors:

Kanamori, Taisei and Yasuda, Shogo and Duan, Runjing and Ohashi, Mei and Amou, Mai and Hori, Kanato and Tsuda, Ryota and Fujimoto, Taiki and Higashi, Kenjirou and Xu, Wei and others,

Journal:

Scientific Reports (2025): 10133

OBP2A regulates epidermal barrier function and protects against cytotoxic small hydrophobic molecules

Authors:

Nakanishi, Shinobu and Hasegawa, Tatsuya and Maeno, Katsuyuki and Motoyama, Akira and Denda, Mitsuhiro

Journal:

iScience (2024)

M$\beta$CD inhibits SFTSV entry by disrupting lipid raft structure of the host cells

Authors:

Cheng, Min and Zhang, Rui and Li, Jianshu and Ma, Wenyuan and Li, Linrun and Jiang, Na and Liu, Bingxin and Wu, Jing and Zheng, Nan and Wu, Zhiwei

Journal:

Antiviral Research (2024): 106004

References

View all 101 references: Citation Explorer

Lowering cholesterol - a review on the role of plant sterols

Authors:

Clifton P., undefined

Journal:

Aust Fam Physician (2009): 218

Prospective studies on the relationship between high-density lipoprotein cholesterol and cardiovascular risk: a systematic review

Authors:

Chirovsky DR, Fedirko V, Cui Y, Sazonov V, Barter P.

Journal:

Eur J Cardiovasc Prev Rehabil (2009): 404

Systematic review and metaanalysis of statins for heterozygous familial hypercholesterolemia in children: evaluation of cholesterol changes and side effects

Authors:

O'Gorman CS, Higgins MF, O'Neill MB.

Journal:

Pediatr Cardiol (2009): 482

Ezetimibe monotherapy for cholesterol lowering in 2,722 people: systematic review and meta-analysis of randomized controlled trials

Authors:

P, undefined and or A, Ara RM, Tumur I, Wilkinson AJ, Paisley S, Duenas A, Durrington PN, Chilcott J.

Journal:

J Intern Med (2009): 568

Association between change in high density lipoprotein cholesterol and cardiovascular disease morbidity and mortality: systematic review and meta-regression analysis

Authors:

Briel M, Ferreira-Gonzalez I, You JJ, Karanicolas PJ, Akl EA, Wu P, Blechacz B, Bassler D, Wei X, Sharman A, Whitt I, Alves da Silva S, Khalid Z, Nordmann AJ, Zhou Q, Walter SD, Vale N, Bhatnagar N, O'Regan C, Mills EJ, Bucher HC, Montori VM, Guyatt GH.

Journal:

Bmj (2009): b92

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Cholesterol Enzyme Mix (lyophilized)	2 bottles
Component D: Cholesterol Standard	1vial (2 mM, 100 µL)
Component E: DMSO	1 vial (200 µL)