Amplite® Colorimetric Aldehyde Quantitation Kit

Amplite® Colorimetric Aldehyde Quantitation Kit is a colorimetric assay kit for detecting aldehydes in a microplate format.

Detection Principle: Uses AldeView™ Yellow to generate a chromogenic product upon reaction with aldehydes.
Sensitivity: Detects as little as 1 nmol of aldehyde in a 100 µL assay volume (10 µM).
Workflow: Compatible with 96-well or 384-well plate format or can be adapted to automation with no separation step.
Instrument Compatibility: Absorbance detection at 550 nm using a clear-bottom microplate.
Applications: Suitable for biological, food, chemical, and environmental research.

Aldehyde dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Aldehyde Quantitation Assay Kit using a Spectrum Max microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
10051	200 Tests	Price

Overview

Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance. There are few reagents or assay kits available for quantifying the amount of aldehydes. Most of existing aldehyde test methods is based on separations either by the tedious and expensive HPLC-MS or GC-MS. Our Amplite® Colorimetric Aldehyde Quantitation kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. Its signal can be easily read by absorbance microplate reader at 550 nm.

Citations

View all 4 citations: Citation Explorer

Biological Activity of Peptide-conjugated Polyion Complex Matrices Consisting of Alginate and Chitosan

Authors:

Fujimori, Chikara and Kumai, Jun and Nakamura, Kyotaro and Gu, Yingzi and Katagiri, Fumihiko and Hozumi, Kentaro and Kikkawa, Yamato and Nomizu, Motoyoshi

Journal:

Peptide Science (2016)

Integrated self-assembling drug delivery system possessing dual responsive and active targeting for orthotopic ovarian cancer theranostics

Authors:

Lin, Chun-Jui and Kuan, Chen-Hsiang and Wang, Li-Wen and Wu, Hsi-Chin and Chen, Yunching and Chang, Chien-Wen and Huang, Rih-Yang and Wang, Tzu-Wei

Journal:

Biomaterials (2016): 12--26

Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice

Authors:

Kumar, Sudhir and Wang, Jiang and Rani, Richa and G, undefined and hi, Ch and rashekhar R, undefined

Journal:

PloS one (2016): e0147864

Fiber-optic protease sensor based on the degradation of thin gelatin films

Authors:

Schyrr, Bastien and Boder-Pasche, Stéphanie and Ischer, Réal and Smajda, Rita and Voirin, Guy

Journal:

Sensing and Bio-Sensing Research (2015): 65--73

References

View all 19 references: Citation Explorer

Measurement of atmospheric hydroxyacetone, glycolaldehyde, and formaldehyde

Authors:

Zhou X, Huang G, Civerolo K, Schwab J.

Journal:

Environ Sci Technol (2009): 2753

Formaldehyde cross-linking of proteins to osteoclast differentiation factor promoter for the identification of biofunctional proteins

Authors:

Haghighi M., undefined

Journal:

Saudi Med J (2009): 472

Methods to reduce formaldehyde levels of cadavers

Authors:

Whitehead MC., undefined

Journal:

Clin Anat (2009): 421

Formaldehyde in China: production, consumption, exposure levels, and health effects

Authors:

Tang X, Bai Y, Duong A, Smith MT, Li L, Zhang L.

Journal:

Environ Int (2009): 1210

Urine formaldehyde level is inversely correlated to mini mental state examination scores in senile dementia

Authors:

Tong Z, Zhang J, Luo W, Wang W, Li F, Li H, Luo H, Lu J, Zhou J, Wan Y, He R.

Journal:

Neurobiol Aging. (2009)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	550 nm
Recommended plate	Clear bottom

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Page updated on October 23, 2026

Component A: AldeView™ Yellow	2 bottles
Component B: Assay Solution	1 bottle (10 mL)
Component C: Aldehyde Standard	1 vial
Component D: Dilution Buffer	1 bottle (20 mL)