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Amplite® Colorimetric Aldehyde Quantitation Kit

Aldehyde dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Aldehyde Quantitation Assay Kit using a Spectrum Max microplate reader (Molecular Devices).
Aldehyde dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Aldehyde Quantitation Assay Kit using a Spectrum Max microplate reader (Molecular Devices).
Aldehyde dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Aldehyde Quantitation Assay Kit using a Spectrum Max microplate reader (Molecular Devices).
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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22


Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance. There are few reagents or assay kits available for quantifying the amount of aldehydes. Most of existing aldehyde test methods is based on separations either by the tedious and expensive HPLC-MS or GC-MS. Our Amplite® Colorimetric Aldehyde Quantitation kit uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. The kit provides a sensitive, one-step colorimetric method to detect as little as 1 nanomole of aldehyde in a 100 µL assay volume (10 µM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. Its signal can be easily read by absorbance microplate reader at 550 nm.


Absorbance microplate reader

Absorbance550 nm
Recommended plateClear bottom


Example protocol


Protocol Summary
  1. Prepare Aldehyde standards and/or test samples (50 µL)
  2. Add 2X AldeView™ Yellow working solution (50 µL)
  3. Incubate at room temperature for 30 to 60 minutes
  4. Monitor absorbance increase at 550 nm 
Important      Thaw all the kit components to room temperature before starting the experiment. Assay solution (Component B) is potentially hazardous. Wear gloves when handling it.


Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Aldehyde standard solution (10 mM)
Add 1 mL of Dilution Buffer (Component D) into the vial of Aldehyde Standard (Component C) to make a 10 mM aldehyde standard solution.


For convenience, use the Serial Dilution Planner:

Aldehyde standard
Take 10 mM Aldehyde standard solution and perform 1:10 dilution to get 1000 uM Aldehyde standard solution (AS7). Then perform 1:3 serial dilutions to get remaining serial dilutions of aldehyde standard (AS6 - AS1).


Add 5 mL of Assay Solution (Component B) into the bottle of AldeView™ Yellow (Component A), and mix well.
Note     5 mL of the 2X AldeView™ Yellow reaction mixture is enough for 1 plate. The reaction mixture is not stable. Use within 2 hours.
Note     Assay solution (Component B) is potentially hazardous. Wear gloves when handling it.


Table 1. Layout of Aldehyde standards and test samples in a white/clear bottom 96-well microplate. AS= Aldehyde Standards (AS1 - AS7, 1 to 1000 µM), BL=Blank Control, TS=Test Samples.
Table 2.Reagent composition for each well.
AS1 - AS750 µLSerial Dilution (1 to 1000 µM)
BL50 µLAssay Buffer
TS50 µLtest sample
  1. Prepare aldehyde standards (AS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 25 µL of reagent per well instead of 50 µL. Note: Both BSA and Tween 20 will interfere with the assay, use less than 0.001% BSA and 0.01% Tween 20 in the samples. 
    Note     If the aldehyde-containing samples are from enzyme reactions such as fructose-1,6-bisphosphate with fructose-1,6-bisphosphate aldolase, prepare 50 µL of enzyme reaction (25 µL for a 384-well plate) as desired. Incubate the enzyme reaction at 37 °C for at least 1 hour. The components of enzyme reaction should be optimized as needed (e.g. an optimized buffer system might be required for a specific enzyme reaction). In most cases, Dilution Buffer (Component D) can also be used for running enzyme reaction if you do not have an optimized enzyme buffer.
  2. Add 50 µL of AldeView™ Yellow working solution to each well of aldehyde standard, blank control, and test samples to make the total aldehyde assay volume of 100 µL/well. For a 384-well plate, add 25 µL of AldeView™ Yellow working solution into each well instead, for a total volume of 50 µL/well.
  3. Incubate the reaction mixture at room temperature for 30 to 60 minutes, protected from light.
  4. Monitor the absorbance increase with an absorbance plate reader at 550 nm.
    Note     Different concentrations of the aldehyde might form different colors with AldeView™ Yellow. 



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