Name: Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Brand: AAT Bioquest
SKU: 10053
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Aldehyde Quantitation Kit

Blue Color

Amplite® Colorimetric Aldehyde Quantitation Kit is a colorimetric assay kit for detecting aldehydes using a mix-and-read microplate assay.

Detection Principle: Uses AldeView™ Blue chemistry to generate a strong absorbance signal in the presence of aldehydes.
Sensitivity: Detects aldehydes down to 0.4 µM.
Signal Response: Produces an absorbance signal proportional to aldehyde concentration.
Workflow: Mix-and-read assay or can be adapted to automation with no separation step.
Applications: Suitable for biological, chemical, food, and environmental research.

Aldehyde dose response was measured in a white wall/clear bottom 96-well plate with Amplite Colorimetric Aldehyde Quantitation Kit using a SpectraMax microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
10053	200 Tests	Price

Overview

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years. Rapid and accurate measurement of aldehydes is an important task for biological research, chemical research, food industry and environmental pollution surveillance. There are a few reagents or assay kits available for quantifying the number of aldehydes. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. Kit 10053 uses a proprietary chromogenic dye that generates a strongly color product upon reacting with an aldehyde. This colorimetric kit provides a sensitive mix-and-read method to detect aldehydes (0.4 uM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Citations

View all 5 citations: Citation Explorer

Aldehyde-mediated protein degradation is responsible for the inhibition of nucleotide excision repair by cigarette sidestream smoke

Authors:

Yang, Guang and Komaki, Yukako and Ibuki, Yuko

Journal:

Mutation Research/Genetic Toxicology and Environmental Mutagenesis (2018)

Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice

Authors:

Kumar, Sudhir and Wang, Jiang and Rani, Richa and G, undefined and hi, Ch and rashekhar R, undefined

Journal:

PloS one (2016): e0147864

Integrated self-assembling drug delivery system possessing dual responsive and active targeting for orthotopic ovarian cancer theranostics

Authors:

Lin, Chun-Jui and Kuan, Chen-Hsiang and Wang, Li-Wen and Wu, Hsi-Chin and Chen, Yunching and Chang, Chien-Wen and Huang, Rih-Yang and Wang, Tzu-Wei

Journal:

Biomaterials (2016): 12--26

Biological Activity of Peptide-conjugated Polyion Complex Matrices Consisting of Alginate and Chitosan

Authors:

Fujimori, Chikara and Kumai, Jun and Nakamura, Kyotaro and Gu, Yingzi and Katagiri, Fumihiko and Hozumi, Kentaro and Kikkawa, Yamato and Nomizu, Motoyoshi

Journal:

Peptide Science (2016)

Fiber-optic protease sensor based on the degradation of thin gelatin films

Authors:

Schyrr, Bastien and Boder-Pasche, Stéphanie and Ischer, Réal and Smajda, Rita and Voirin, Guy

Journal:

Sensing and Bio-Sensing Research (2015): 65--73

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	620 nm
Recommended plate	Clear bottom

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Page updated on October 28, 2026

Component A: AldeView™ Blue	2 bottles
Component B: Assay Buffer	1 bottle (25 mL)
Component C: AldeView™ Blue Enhancer	1 bottle (10 mL)
Component D: Aldehyde Standard	1 vial