Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*

Amplite® Colorimetric Alkaline Phosphatase Assay Kit

Yellow Color

Amplite® Colorimetric Alkaline Phosphatase Assay Kit is a colorimetric assay kit for quantification of alkaline phosphatase (ALP) activity in solutions, cell extracts, and solid surfaces.

Detection Principle: Uses pNPP to generate a yellow product measurable at ~400 nm.
Sensitivity: Detects ALP concentrations as low as 0.3 mU/mL after 30 minutes.
Specificity: Uses pNPP as a chromogenic phosphatase substrate for activity measurement.
Workflow: Compatible with 96- or 384-well microplates and HTS liquid handling systems.
Applications: Suitable for ALP activity measurements in immunoassays, cell extracts, and blotting workflows.

Alkaline phosphatase dose response was measured with the Amplite® Colorimetric Alkaline Phosphatase Assay Kit in a white/clear bottom 96-well plate using a NovoStar microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
11950	500 Tests	Price

Overview

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses pNPP, a chromogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts as well as on solid surfaces (such as PVDF membranes). The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

Citations

View all 28 citations: Citation Explorer

Melatonin Rescues Enamel Defects Induced by Maternal Circadian Disruption via Targeting the BMAL1-JNK3 Axis

Authors:

Wang, Xuanyu and Liu, Jing and Bi, Mengning and Li, Xue and Yang, Xuejiao and Xu, Wenke and Chen, Zhihua and Wang, Yueying and Ji, Fang

Journal:

International Dental Journal (2026): 109673

Micro-arranged ZnO particles and conductive fillers in PCL composites for enhanced piezoelectric and dielectric properties in bone tissue engineering applications

Authors:

Fern{\'a}ndez-Gil, Francisco and Olate-Moya, Felipe and Aguilar-Cosme, Jos{\'e} Ricardo and Garcia-Molleja, Javier and Fern{\'a}ndez-Bl{\'a}zquez, Juan Pedro and Cartmell, Sarah and Palza, Humberto

Journal:

Materials \& Design (2025): 113672

Evaluating the holistic intestinal biological efficacy of different lettuce lines

Authors:

Heming, Bo

Bioengineered intestinal tubules as a tool to test intestinal biological efficacy of lettuce species

Authors:

Jochems, Paulus GM and Heming, Bo and Lapin, Dmitry and Moonen, Naomi EL and Van den Ackerveken, Guido and Masereeuw, Rosalinde

Journal:

npj Science of Food (2022): 1--8

MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis

Authors:

Hayashi, Yasutaka and Kawabata, Kimihito C and Tanaka, Yosuke and Uehara, Yasufumi and Mabuchi, Yo and Murakami, Koichi and Nishiyama, Akira and Kiryu, Shigeru and Yoshioka, Yusuke and Ota, Yasunori and others,

Journal:

Cell Reports (2022): 110805

References

View all 109 references: Citation Explorer

8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline phosphatase

Authors:

Zhu X, Jiang C.

Journal:

Clin Chim Acta. (2006)

Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines

Authors:

Lee DH, Lim BS, Lee YK, Yang HC.

Journal:

Cell Biol Toxicol (2006): 39

The effect of alkaline phosphatase inhibitors on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue

Authors:

Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ.

Journal:

Ann Clin Biochem (2006): 207

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Authors:

Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ.

Journal:

Clin Chim Acta (2005): 101

Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study

Authors:

Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B.

Journal:

Acta Biochim Pol (2004): 189

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	400 nm
Recommended plate	Clear bottom

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Page updated on October 28, 2026

Component A: pNPP (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Alkaline Phosphatase Standard	1 vial (lyophilized powder, 10 units)