Amplite® Colorimetric α-Ketoglutarate Quantitation Kit

Amplite® Colorimetric α-Ketoglutarate Quantitation Kit is a colorimetric assay kit for quantifying α-ketoglutarate in biological samples through an enzyme-coupled hydrogen peroxide readout.

Colorimetric detection: Uses Amplite® Red to detect hydrogen peroxide generated by an enzyme-coupled reaction.
Quantitative range: Supports α-ketoglutarate standards from 1.563 to 100 µM.
Enzyme-coupled chemistry: Converts α-ketoglutarate into a proportional hydrogen peroxide signal.
Microplate compatible: Read absorbance at 570 nm using a clear-bottom 96-well plate.
Applications: Suitable for α-ketoglutarate quantitation in metabolic and other biological sample studies.

Alpha-ketoglutarate dose response was measured with Amplite® Colorimetric α-Ketoglutarate Quantitation Kit in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices) with path check on mode.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10085	200 Tests	Price

Overview

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism. As a precursor of glutamate and glutamine, α-ketoglutarate is a central metabolic fuel for cells of the gastrointestinal tract as well. It can decrease protein catabolism and increase protein synthesis to enhance bone tissue formation in the skeletal muscles and can be used in clinical applications. Alpha-ketoglutarate is used for kidney disease; intestinal and stomach disorders, including bacterial infections; liver problems; cataracts; and recurring yeast infections. It is also used for improving the way kidney patients receiving hemodialysis treatments process protein. AAT Bioquest's Amplite® Colorimetric α-Ketoglutarate Quantitation Kit offers a sensitive colorimetric assay for quantifying α-ketoglutarate in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected by Amplite® Red in an absorbance microplate reader at 570 nm.

Citations

View all 2 citations: Citation Explorer

Sustained therapeutic effects of self-assembled hyaluronic acid nanoparticles loaded with $\alpha$-Ketoglutarate in various osteoarthritis stages

Authors:

Wang, Xinli and Xue, Yufei and Hao, Kaili and Peng, Bo and Chen, Hongli and Liu, Hui and Wang, Jing and Cao, Jiahao and Dong, Wengang and Zhang, Siqi and others,

Journal:

Biomaterials (2024): 122845

Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes

Authors:

Jia, Yinzhao and Yin, Chuanzheng and Ke, Wenbo and Liu, Jing and Guo, Bing and Wang, Xiaofei and Zhao, Peng and Hu, Shaobo and Zhang, Chen and Li, Xuan and others,

Journal:

Science of The Total Environment (2023): 163069

References

View all 40 references: Citation Explorer

Loss of Nardilysin, a Mitochondrial Co-chaperone for alpha-Ketoglutarate Dehydrogenase, Promotes mTORC1 Activation and Neurodegeneration

Authors:

Yoon WH, S and oval H, Nagarkar-Jaiswal S, Jaiswal M, Yamamoto S, Haelterman NA, Putluri N, Putluri V, Sreekumar A, Tos T, Aksoy A, Donti T, Graham BH, Ohno M, Nishi E, Hunter J, Muzny DM, Carmichael J, Shen J, Arboleda VA, Nelson SF, Wangler MF, Karaca E, Lupski JR, Bellen HJ.

Journal:

Neuron (2017): 115

Dietary alpha-ketoglutarate promotes higher protein and lower triacylglyceride levels and induces oxidative stress in larvae and young adults but not in middle-aged Drosophila melanogaster

Authors:

Bayliak MM, Lylyk MP, Shmihel HV, Sorochynska OM, Semchyshyn OI, Storey JM, Storey KB, Lushchak VI.

Journal:

Comp Biochem Physiol A Mol Integr Physiol (2017): 28

The facial triad in the alpha-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation

Authors:

Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ.

Journal:

J Inorg Biochem (2017): 26

Increased Circulating Levels of Alpha-Ketoglutarate in Morbidly Obese Women with Non-Alcoholic Fatty Liver Disease

Authors:

Aragones G, Auguet T, Berlanga A, Guiu-Jurado E, Martinez S, Armengol S, Sabench F, Ras R, Hern and ez M, Aguilar C, Colom J, Sirvent JJ, Del Castillo D, Richart C.

Journal:

PLoS One (2016): e0154601

Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward alpha-Ketoglutarate

Authors:

Martin-Mora D, Ortega A, Reyes-Darias JA, Garcia V, Lopez-Farfan D, Matilla MA, Krell T.

Journal:

Front Microbiol (2016): 1937

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom
Instrument specification(s)	Path check

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2026

Component A: Amplite® Red	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: α-Ketoglutarate Standard	1 vial (10 mM, 100 µL)
Component E: DMSO	1 vial (100 µL)