Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*

Amplite® Colorimetric Ammonia Quantitation Kit

Blue Color

Ammonia is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. It is produced in liver and converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). The determination of ammonia is very useful test in clinical laboratory to monitor health status. Our Amplite® Colorimetric Ammonia Assay Kit provides a simple and sensitive colorimetric method for the quantitation of ammonia concentration in foods and biological samples such as serum, plasma and urine, etc. The assay is based on an enzyme-coupled reaction of ammonia in the assay buffer, and finally produces a blue colored product. The intensity of color produced is proportional to the concentration of ammonia in the sample, which can be measured colorimetrically at 660-670 nm. This Amplite® Colorimetric Ammonia Assay Kit provides a simple assay to detect ammonia. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Ammonia dose response on a 96-well white clear bottom plate using a Spectrum Max microplate reader (Molecular Devices) measured with Amplite® Colorimetric Ammonia/Ammonium Quantitation Kit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10059	200 Tests	Price

Citations

View all 6 citations: Citation Explorer

Biology-aware machine learning for culture medium optimization

Authors:

Hashizume, Takamasa and Ying, Bei-Wen

Journal:

New Biotechnology (2025)

A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster

Authors:

Yoshinari, Yuto and Nishimura, Takashi and Yoshii, Taishi and Kondo, Shu and Tanimoto, Hiromu and Kobayashi, Tomoe and Matsuyama, Makoto and Niwa, Ryusuke

Journal:

Nature Communications (2024): 10819

Hepatic ketone body regulation of renal gluconeogenesis

Authors:

Hatano, Ryo and Lee, Eunyoung and Sato, Hiromi and Kiuchi, Masahiro and Hirahara, Kiyoshi and Nakagawa, Yoshimi and Shimano, Hitoshi and Nakayama, Toshinori and Tanaka, Tomoaki and Miki, Takashi

Journal:

Molecular Metabolism (2024): 101934

A high-protein diet-responsive gut hormone regulates behavioural and metabolic optimization in Drosophila melanogaster

Authors:

Niwa, Ryusuke and Yoshinari, Yuto and Nishimura, Takashi and Yoshii, Taishi and Kondo, Shu and Tanimoto, Hiromu and Kobayashi, Tomoe and Matsuyama, Makoto

High-Aspect-Ratio SU-8-Based Optofluidic Device for Ammonia Detection in Cell Culture Media

Authors:

Dervisevic, Esma and Voelcker, Nicolas H and Risbridger, Gail and Tuck, Kellie L and Cadarso, Victor J

Journal:

ACS sensors (2020)

References

View all 173 references: Citation Explorer

Systemic inflammation and ammonia in hepatic encephalopathy

Authors:

Tranah TH, Vijay GK, Ryan JM, Shawcross DL.

Journal:

Metab Brain Dis (2013): 1

High pleural ammonia negatively interferes with the measurement of adenosine deaminase activity

Authors:

Loh TP, Tan KM, Chew S, Chan DS.

Journal:

BMJ Case Rep (2013)

Role of branched chain amino acids in cerebral ammonia homeostasis related to hepatic encephalopathy

Authors:

Bak LK, Waagepetersen HS, Sorensen M, Ott P, Vilstrup H, Keiding S, Schousboe A.

Journal:

Metab Brain Dis. (2013)

Catalytic mechanisms and biocatalytic applications of aspartate and methylaspartate ammonia lyases

Authors:

de Villiers M, Puthan Veetil V, Raj H, de Villiers J, Poelarends GJ.

Journal:

ACS Chem Biol (2012): 1618

Multiple functions of the crustacean gill: osmotic/ionic regulation, acid-base balance, ammonia excretion, and bioaccumulation of toxic metals

Authors:

Henry RP, Lucu C, Onken H, Weihrauch D.

Journal:

Front Physiol (2012): 431

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Absorbance microplate reader
Absorbance	660 - 670 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2025

Component A: Assay Buffer I	1 bottle (10 mL)
Component B: Assay Buffer II	1 bottle (10 mL)
Component C: Ammonium Chloride Standard (1.0 M)	1 vial (0.2mL)