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Amplite® Colorimetric Antioxidant Activity Assay Kit
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism. An excessive level of ROS damages cellular components, which links to numerous diseases, including cancer, metabolic disorders, neurodegenerative disease, etc. Antioxidants, including enzymes, macromolecules, and small molecules, are generated to combat the ROS-induced damages in living organisms. Quantitative analysis of the antioxidant activity in body fluids, tissues, and cells provides important biological information. Our Amplite® Colorimetric Antioxidant Assay Kit uses ABTS as a colorimetric indicator of antioxidant activity based on the observation that the ability of antioxidants to prevent the oxidation of ABTS (2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) to ABTS+ by Horseradish Peroxidase (HRP) and Hydrogen peroxide (H2O2). ABTS+, a soluble chromogen, can be monitored spectrophotometrically at 405 nm.
Trolox dose-response was measured with the Amplite® Colorimetric Antioxidant Activity Assay Kit (Cat# 15900) in a 96-well clear plate using a SpectraMax microplate reader (Molecular Devices).
Trolox dose-response was measured with the Amplite® Colorimetric Antioxidant Activity Assay Kit (Cat# 15900) in a 96-well clear plate using a SpectraMax microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
15900200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Absorbance microplate reader
Absorbance405 nm
Recommended plateClear bottom
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Page updated on October 13, 2025