Name: Amplite® Colorimetric Antioxidant Activity Assay Kit
Brand: AAT Bioquest
SKU: 15900
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Antioxidant Activity Assay Kit

Amplite® Colorimetric Antioxidant Activity Assay Kit is a colorimetric assay kit for quantifying antioxidant activity in body fluids, tissues, and cells using an ABTS-based microplate assay.

Colorimetric detection: Measures antioxidant-dependent suppression of ABTS+ formation at 405 nm.
ABTS-based chemistry: Uses HRP and hydrogen peroxide to oxidize ABTS, with antioxidants inhibiting the reaction.
Rapid workflow: Provides a colorimetric readout after approximately 5 minutes of incubation.
Microplate compatible: Designed for absorbance microplate readers using clear-bottom plates.
Applications: Suitable for measuring antioxidant activity in body fluids, tissues, cells, and related biological samples.

Trolox dose-response was measured with the Amplite® Colorimetric Antioxidant Activity Assay Kit (Cat# 15900) in a 96-well clear plate using a SpectraMax microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15900	200 Tests	Price

Overview

Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism. An excessive level of ROS damages cellular components, which links to numerous diseases, including cancer, metabolic disorders, neurodegenerative disease, etc. Antioxidants, including enzymes, macromolecules, and small molecules, are generated to combat the ROS-induced damages in living organisms. Quantitative analysis of the antioxidant activity in body fluids, tissues, and cells provides important biological information. Our Amplite® Colorimetric Antioxidant Assay Kit uses ABTS as a colorimetric indicator of antioxidant activity based on the observation that the ability of antioxidants to prevent the oxidation of ABTS (2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) to ABTS+ by Horseradish Peroxidase (HRP) and Hydrogen peroxide (H2O2). ABTS+, a soluble chromogen, can be monitored spectrophotometrically at 405 nm.

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	405 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2026

Component A: Horseradish Peroxidase	1 vial (lyophylized powder)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: ReadiUse™ ABTS Substrate Solution	1 bottle (35 mL)
Component D: Trolox	1 vial (2.5 mg)