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Amplite® Colorimetric Asparaginase Activity Assay Kit
The Amplite® Colorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples. Asparaginase activity is determined by a coupled enzyme assay, which results in the formation of a colored product with absorbance at 575 nm. The amount of the colored product and the absorbance value is proportional to the aspartate generated by the asparaginase enzyme. One unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. Asparaginase is an essential enzyme catalyzing the hydrolysis of non-essential amino acid Asparagine to Aspartate and Ammonia. It plays a crucial role in cellular functions, particularly in hematopoietic cells which rely on exogenous asparagine for protein synthesis. Asparaginase is found in plants, microorganisms, and certain animals, but does not occur naturally in humans, making it a valuable therapeutic agent in medicine and an essential tool in various industries. Asparaginase is used to treat acute lymphocytic leukemia (ALL) by starving tumor cells of needed nutrients and slowing tumor cell growth. Depletion of circulating asparagine by asparaginase induces cell cycle arrest and apoptosis in malignant cells, offering a targeted approach to cancer treatment. Beyond its medical applications, asparaginase is also used in the food industry to reduce the formation of acrylamide, a potentially carcinogenic compound, in starchy and fried foods. The versatility of this enzyme extends to various research fields, including biotechnology and biochemistry.
Aspartate dose responses was measured using the Amplite® Colorimetric Asparaginase Activity Assay Kit on a 96-well clear bottom microplate with a ClarioStar microplate reader (BMG) at 570 nm.
Aspartate dose responses was measured using the Amplite® Colorimetric Asparaginase Activity Assay Kit on a 96-well clear bottom microplate with a ClarioStar microplate reader (BMG) at 570 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11800100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance570 nm
Recommended plateClear bottom
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Page updated on March 5, 2026