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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, thus deactivates the molecule's antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance. AAT Bioquest's Colorimetric Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The β-lactamase activity is detected using Nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.
β-Lactamase dose response was measured with Amplite™ Colorimetric Beta-Lactamase Assay Kit (Cat#12551) on a 96-well clear bottom plate using a SpectraMax reader (Molecular Devices). 
β-Lactamase dose response was measured with Amplite™ Colorimetric Beta-Lactamase Assay Kit (Cat#12551) on a 96-well clear bottom plate using a SpectraMax reader (Molecular Devices). 
CatalogSize
Price
Quantity
12551200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Absorbance microplate reader
Absorbance490/380 nm
Recommended plateClear bottom
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Page updated on September 25, 2025