Name: Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
Brand: AAT Bioquest
SKU: 12551
Price: 475 USD
Availability: InStock

Amplite® Colorimetric Beta-Lactamase Activity Assay Kit

Amplite® Colorimetric Beta-Lactamase Activity Assay Kit is a colorimetric assay kit for quantification of beta-lactamase activity in biological samples.

Detection Principle: Nitrocefin changes from yellow to red upon beta-lactamase hydrolysis.
Quantitation Range: β-Lactamase standards span 7.8–500 µU/mL.
Workflow: Incubates 30–60 minutes at room temperature.
Instrument Compatibility: Reads the 490/380 nm absorbance ratio.
Applications: Suitable for beta-lactamase activity, antibiotic resistance, and inhibitor studies.

&beta;-Lactamase dose response was measured with Amplite&trade; Colorimetric Beta-Lactamase Assay Kit (Cat#12551) on a 96-well clear bottom plate using a SpectraMax reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
12551	200 Tests	Price

Overview

β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, thus deactivates the molecule's antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance. AAT Bioquest's Colorimetric Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The β-lactamase activity is detected using Nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.

Citations

View all 2 citations: Citation Explorer

Daily rhythms modulate Acinetobacter baumannii physiology impacting infection outcome and antibiotic-inactivating capacity

Authors:

Giordano, Roc{\'\i}o Anabel and Permingeat, Valent{\'\i}n and Mora, B{\'a}rbara Perez and Arana, Natalia and Braccialarghe, Tom{\'a}s and Fusini, Mat{\'\i}as and Serra, Diego and Migliori, Mar{\'\i}a Laura and Golombek, Diego Andr{\'e}s and Perez, Ana Rosa and others,

Circadian rhythms in the critical human pathogen Acinetobacter baumannii

Authors:

Mussi, Mar{\'\i}a Alejandra and Permingeat, Valent{\'\i}n and Mora, B{\'a}rbara Perez and Migliori, Mar{\'\i}a and Arana, Natalia and Fern{\'a}ndez, Julia and Allasia, Mar{\'\i}a Bel{\'e}n and Lamberti, Melisa and Di Venanzio, Gisela and Feldman, Mario and others,

References

View all 14 references: Citation Explorer

A nitrocefin-based amperometric assay for the rapid quantification of extended-spectrum beta-lactamase-producing Escherichia coli in wastewaters

Authors:

Chantemesse B, Betelli L, Solanas S, Vienney F, Bollache L, Hartmann A, Rochelet M.

Journal:

Water Res (2017): 375

Amperometric detection of extended-spectrum beta-lactamase activity: application to the characterization of resistant E. coli strains

Authors:

Rochelet M, Solanas S, Betelli L, Neuwirth C, Vienney F, Hartmann A.

Journal:

Analyst (2015): 3551

Complete (1)H, (1)(5)N, and (1)(3)C resonance assignments of Bacillus cereus metallo-beta-lactamase and its complex with the inhibitor R-thiomandelic acid

Authors:

Karsisiotis AI, Damblon C, Roberts GC.

Journal:

Biomol NMR Assign (2014): 313

Evaluation of phenotypic tests for the detection of AmpC beta-lactamase in clinical isolates of Escherichia coli

Authors:

H, undefined and a D, P and ey A, Asthana AK, Rawat A, H and a S, Thakuria B.

Journal:

Indian J Pathol Microbiol (2013): 135

An altered zinc-binding site confers resistance to a covalent inactivator of New Delhi metallo-beta-lactamase-1 (NDM-1) discovered by high-throughput screening

Authors:

Thomas PW, Spicer T, Cammarata M, Brodbelt JS, Hodder P, Fast W.

Journal:

Bioorg Med Chem (2013): 3138

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	490/380 nm
Recommended plate	Clear bottom

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Page updated on October 16, 2026

Component A: Nitrocefin	1 vial (100 µL)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: β-Lactamase Standard	1 vial (lyophilized powder)