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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

BSA dose responses were measured with Amplite® Colorimetric Bradford Protein Quantitation Assay Kit using a clear bottom 96-well plate.
BSA dose responses were measured with Amplite® Colorimetric Bradford Protein Quantitation Assay Kit using a clear bottom 96-well plate.
Ordering information
Price ()
Catalog Number11118
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22


The traditional Bradford protein assay is widely used for quantifying protein concentrations. However, many of the commercial protocols are complicated. Amplite®™ Colorimetric Braford Protein Quantitation Assay Kit is a two-component and detergent-compatible assay to determine total protein concentrations. The assay is based on the same Coomassie Blue G-250 protein indicator as Bradford protein assay and provides comparable accuracy. Our proprietary formulation makes our kit much more convenient and rapid. The protein signal is monitored around 600 nm and assay is completed within 30 minutes. Amplite®™ Colorimetric Braford Protein Quantitation Assay Kit can be performed in a convenient 96-well microtiter-plate format and easily adapted to automation with no separation steps required.


Absorbance microplate reader

Absorbance595 nm
Recommended plateClear bottom


Component A: Bradford Assay Solution50 mL
Component B: BSA Standard (1 mg/mL)1 mL

Example protocol


Protocol summary
  1. Prepare Bradford working solution (50 μL)
  2. Add BSA standards or test samples (50 μL)
  3. Incubate at room temperature for 5 - 15 minutes
  4. Read absorbance at 595 nm 

Thaw all the kit components at room temperature before use.


For convenience, use the Serial Dilution Planner:

BSA Standard
Add 20 µL of 1 mg/mL BSA Standard (Component C) to 480 µL of PBS (not provided) to generate 40 µg/mL BSA standard solution (BS1). Then perform 1:2 serial dilutions in PBS to get serially diluted BSA standards BS2 - BS7. Note: It is necessary to create a standard curve for each assay.


Table 1.Layout of BSA standards and test samples in a clear bottom 96-well microplate. BS= BSA Standards (BS1 - BS7, 40 to 0.625 µg/mL); BL=Blank Control; TS=Test Samples
BS2 BS2 ... ...
BS3 BS3    
BS4 BS4    
BS5 BS5    
BS6 BS6    
BS7 BS7    
BL BL    
Table 2.Reagent composition for each well
Well Volume Reagent
BS1-BS7 50 µL Serial Dilutions (40-0.625 µg/mL)
BL 50 µL PBS
TS 50 µL Test Samples
  1. Prepare BSA standards (BS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2.
  2. Add 50 µL of Bradford working solution to each well of BSA standard, blank control, and test samples to make the total assay volume of 100 µL/well.
  3. Incubate the reaction at room temperature for 5 to 15 minutes.
  4. Read absorbance with an absorbance microplate reader at OD 595 nm. 


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Journal: Frontiers in bioengineering and biotechnology (2019): 452
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Journal: Breast cancer (Dove Medical Press) (2019): 99-110
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