Name: Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit
Brand: AAT Bioquest
SKU: 11406
Price: 594 USD
Availability: InStock

Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit is a colorimetric assay kit for detecting butyrylcholinesterase (BChE) activity using a butyrylthiocholine-based substrate.

Detection Principle: DTNB reacts with generated thiocholine to produce a yellow chromophore.
Sensitivity: Detects BChE activity as low as 6 mU/mL after 10 minutes.
Specificity: Uses butyrylthiocholine for enzyme activity measurement.
Instrument Compatibility: Read absorbance at 410 ± 5 nm using a clear-bottom plate.
Applications: Suitable for BChE activity measurements and enzyme-inhibition studies.

Butyrylcholinesterase dose response was measured in a white/clear bottom 96-well plate with Amplite® Colorimetric Butyrylcholinesterase Assay Kit using a SpectraMax microplate reader. As low as 6.0 mU/mL of Butyrylcholinesterase can be detected with 10 minutes incubation (n=3).

Catalog	Size	Price	Quantity
11406	100 Tests	Price

Overview

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized in liver and present in blood. BChE is a nonspecific cholinesterase enzyme and can hydrolyze many different choline esters, serving as the first line of defense against toxic compounds reaching the bloodstream. It has been identified as a clinical biomarker of organophosphate poisoning. Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit is based on a synthetic butyrylthiocholine-based substrate, which can be hydrolyzed by BChE and produce thiocholine. Thiocholine can react with 5, 5'-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be detected at 410 nm. The assay is convenient, sensitive and can detect as low as 6 mU/mL in variety of samples.

Citations

View all 37 citations: Citation Explorer

Cerebrotendinous xanthomatosis--three patients, three faces

Authors:

Schumann, F and Steinhagen-Thiessen, E and Kassner, U

Journal:

Atherosclerosis (2021): e182--e183

Identification of Compounds for Butyrylcholinesterase Inhibition

Authors:

Li, Shuaizhang and Li, Andrew J and Travers, Jameson and Xu, Tuan and Sakamuru, Srilatha and Klumpp-Thomas, Carleen and Huang, Ruili and Xia, Menghang

Journal:

SLAS DISCOVERY: Advancing the Science of Drug Discovery (2021): 24725552211030897

Structure-activity study of fluorine or chlorine-substituted cinnamic acid derivatives with tertiary amine side chain in acetylcholinesterase and butyrylcholinesterase inhibition

Authors:

Gao, X. H., Tang, J. J., Liu, H. R., Liu, L. B., Liu, Y. Z.

Journal:

Drug Dev Res (2019): 438-445

Profiling Auspicious Butyrylcholinesterase Inhibitory Activity of Two Herbal Molecules: Hyperforin and Hyuganin C

Authors:

Orhan, I. E., Senol Deniz, F. S., Traedal-Henden, S., Ceron-Carrasco, J. P., den Haan, H., Pena-Garcia, J., Perez-Sanchez, H., Emerce, E., Skalicka-Wozniak, K.

Journal:

Chem Biodivers (2019): e1900017

Haplotypes of butyrylcholinesterase K-variant and their influence on the enzyme activity

Authors:

Jasiecki, J., Zuk, M., Krawczynska, N., Jonca, J., Szczoczarz, A., Lew and owski, K., Waleron, K., Wasag, B.

Journal:

Chem Biol Interact (2019): 154-157

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	410 ± 5 nm
Recommended plate	Clear bottom

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Page updated on October 28, 2026

Component A: DTNB	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Butyrylthiocholine	1 vial
Component D: Butyrylcholinesterase Standard	1 vial (1 U)