Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*

Amplite® Colorimetric Calcium Quantitation Kit

Blue Color

Amplite® Colorimetric Calcium Quantitation Kit *Blue Color* is a colorimetric assay kit for detecting calcium in physiological solutions using Calcium Blue™ as a chromogenic calcium indicator.

Colorimetric detection: Calcium Blue™ forms an intensely absorbing complex with calcium.
High sensitivity: Detects Ca²⁺ concentrations as low as approximately ~2.5 µM after 5 minutes.
Calcium-binding chemistry: Calcium Blue™ binds calcium tightly in the neutral pH range.
Microplate compatible: Read absorbance at 600 or 650 nm using a clear-bottom plate.
Applications: Suitable for calcium quantitation in physiological solutions and calcium-related research.

Calcium dose response was measured on a 96-well black wall/clear bottom plate with the Amplite® Colorimetric Calcium Quantitation Kit. As low as ~ 2.5 µM Ca2+ was detected with 5 minutes incubation time (n=3)

Protocol

SDS

Catalog	Size	Price	Quantity
36361	200 Tests	Price

Overview

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca2+ into and out of the cytoplasm functions as a signal for many cellular processes. Calcium is the fifth most abundant element by mass in the human body, where it is a common cellular ionic messenger with many functions, and serves also as a structural element in bone. Calcium plays an important role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. The serum level of calcium is closely regulated within a fairly limited range (9 to 10.5 mg/dL) in the human body. Both hypocalcaemia and hypercal caemia are serious medical disorders. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. Amplite® Calcium Detection Kit provides a simple method for detecting calcium in physiology solutions. This kit uses our Calcium Blue™ as the chromogenic calcium indicator. Its absorbance changes in response to calcium binding. Calcium Blue™ binds calcium tightly in the neutral pH range, generating Calcium Blue™-calcium complex that has intense absorption at ~650 nm.

Citations

View all 5 citations: Citation Explorer

Repurposing SKQ1 as a meropenem potentiator against NDM-1-producing Escherichia coli

Authors:

Li, Xiaodan and Zhang, Ziyi and Zhang, Zhaoran and Liu, Di and Lu, Hao and Lu, Wenjia and Qian, Yulin and Chen, Huanchun and Wang, Chenchen and Tan, Chen

Journal:

Virulence (2026): 2728436

Mechanism of triterpenoid saponin mediated augmentation of saporin based immunotoxin cytotoxicity

Authors:

Wensley, Harrison James

The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase

Authors:

Ma, Bo and Fang, Chao and Lu, Linshan and Wang, Mingzhi and Xue, Xiaoyan and Zhou, Ying and Li, Mingkai and Hu, Yue and Luo, Xiaoxing and Hou, Zheng

Journal:

Nature communications (2019): 1--11

Microcystin-LR causes sexual hormone disturbance in male rat by targeting gonadotropin-releasing hormone neurons

Authors:

Wang, Xueting and Ding, Jie and Xiang, Zou and Jiang, Peipei and Du, Jing and Han, Xiaodong

Journal:

Toxicon (2016): 45--55

High efficiency single-step biomaterial-based microparticle fabrication via template-directed supramolecular coordination chemistry

Authors:

Lai, Kwok Kei and Renneberg, Reinhard and Mak, Wing Cheung

Journal:

Green Chemistry (2016): 1715--1723

References

View all 105 references: Citation Explorer

Calcium, magnesium, iron, zinc and copper concentration in the hair of tobacco smokers

Authors:

Unkiewicz-Winiarczyk A, Bagniuk A, Gromysz-Kalkowska K, Szubartowska E.

Journal:

Biol Trace Elem Res (2009): 152

Genetic and nongenetic variation in concentration of selenium, calcium, potassium, zinc, magnesium, and phosphorus in milk of Dutch Holstein-Friesian cows

Authors:

van Hulzen KJ, Sprong RC, van der Meer R, van Arendonk JA.

Journal:

J Dairy Sci (2009): 5754

Comparison of calcium, magnesium, sodium, potassium, zinc, and creatinine concentration in 24-h and spot urine samples in women

Authors:

Ilich JZ, Blanusa M, Orlic ZC, Orct T, Kostial K.

Journal:

Clin Chem Lab Med (2009): 216

Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators

Authors:

Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD.

Journal:

J Endod (2009): 727

3,4-Dichloropropionanilide (DCPA) inhibits T-cell activation by altering the intracellular calcium concentration following store depletion

Authors:

Lewis TL, Brundage KM, Brundage RA, Barnett JB.

Journal:

Toxicol Sci (2008): 97

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	600 or 650 nm
Recommended plate	Clear bottom

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Page updated on September 29, 2026

Component A: Calcium Blue™	1 bottle (10 mL)
Component B: Dilution Buffer	1 bottle (20 mL)
Component C: 300 mM Calcium Standard	1 vial (250 µL)