Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*

Amplite® Colorimetric Calcium Quantitation Kit

Blue Color

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca2+ into and out of the cytoplasm functions as a signal for many cellular processes. Calcium is the fifth most abundant element by mass in the human body, where it is a common cellular ionic messenger with many functions, and serves also as a structural element in bone. Calcium plays an important role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. The serum level of calcium is closely regulated within a fairly limited range (9 to 10.5 mg/dL) in the human body. Both hypocalcaemia and hypercal caemia are serious medical disorders. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. Amplite® Calcium Detection Kit provides a simple method for detecting calcium in physiology solutions. This kit uses our Calcium Blue™ as the chromogenic calcium indicator. Its absorbance changes in response to calcium binding. Calcium Blue™ binds calcium tightly in the neutral pH range, generating Calcium Blue™-calcium complex that has intense absorption at ~650 nm.

Calcium dose response was measured on a 96-well black wall/clear bottom plate with the Amplite® Colorimetric Calcium Quantitation Kit. As low as ~ 2.5 µM Ca2+ was detected with 5 minutes incubation time (n=3)

Protocol

SDS

COA

Catalog	Size	Price	Quantity
36361	200 Tests	Price

Citations

View all 4 citations: Citation Explorer

Mechanism of triterpenoid saponin mediated augmentation of saporin based immunotoxin cytotoxicity

Authors:

Wensley, Harrison James

The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase

Authors:

Ma, Bo and Fang, Chao and Lu, Linshan and Wang, Mingzhi and Xue, Xiaoyan and Zhou, Ying and Li, Mingkai and Hu, Yue and Luo, Xiaoxing and Hou, Zheng

Journal:

Nature communications (2019): 1--11

Microcystin-LR causes sexual hormone disturbance in male rat by targeting gonadotropin-releasing hormone neurons

Authors:

Wang, Xueting and Ding, Jie and Xiang, Zou and Jiang, Peipei and Du, Jing and Han, Xiaodong

Journal:

Toxicon (2016): 45--55

High efficiency single-step biomaterial-based microparticle fabrication via template-directed supramolecular coordination chemistry

Authors:

Lai, Kwok Kei and Renneberg, Reinhard and Mak, Wing Cheung

Journal:

Green Chemistry (2016): 1715--1723

References

View all 105 references: Citation Explorer

Calcium, magnesium, iron, zinc and copper concentration in the hair of tobacco smokers

Authors:

Unkiewicz-Winiarczyk A, Bagniuk A, Gromysz-Kalkowska K, Szubartowska E.

Journal:

Biol Trace Elem Res (2009): 152

Genetic and nongenetic variation in concentration of selenium, calcium, potassium, zinc, magnesium, and phosphorus in milk of Dutch Holstein-Friesian cows

Authors:

van Hulzen KJ, Sprong RC, van der Meer R, van Arendonk JA.

Journal:

J Dairy Sci (2009): 5754

Comparison of calcium, magnesium, sodium, potassium, zinc, and creatinine concentration in 24-h and spot urine samples in women

Authors:

Ilich JZ, Blanusa M, Orlic ZC, Orct T, Kostial K.

Journal:

Clin Chem Lab Med (2009): 216

Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators

Authors:

Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD.

Journal:

J Endod (2009): 727

3,4-Dichloropropionanilide (DCPA) inhibits T-cell activation by altering the intracellular calcium concentration following store depletion

Authors:

Lewis TL, Brundage KM, Brundage RA, Barnett JB.

Journal:

Toxicol Sci (2008): 97

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	600 or 650 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025

Component A: Calcium Blue™	1 bottle (10 mL)
Component B: Dilution Buffer	1 bottle (20 mL)
Component C: 300 mM Calcium Standard	1 vial (250 µL)