Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Amplite® Colorimetric Caspase 3/7 Assay Kit

Yellow Color

Caspases play important roles in apoptosis and cell signaling. The activation of Caspase 3/7 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3/7 is also identified as a drug-screening target. Caspase inhibitors have anti-cancer and other pharmalogical potentials. It has been proven that Caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). Our Amplite® Colorimetric Caspase 3/7 Assay Kit uses (Z-DEVD)2R110 as the chromogenic indicator for assaying caspase 3/7 activity. R110 peptide sustrates are colorless. Cleavage of R110 peptides by caspases generates R110, a yellow color dye that can be monitored at 490-520 nm. The increase in the absorbance of caspase-induced R110 is proportional to the activities of caspases. This kit can be used to continuously measure the activities of caspase 3/7 in cell extracts and purified enzyme preparations with an absorbance microplate reader with much higher sensitivity than the other commercial kits that use DEVD-pNA peptide.

<p>Detection of caspase 3/7 Activity in Jurkat cells. The cells were treated with staurosporine (SS) at the concentration of 0-1 µM for 4 hours at 37ºC. After treatment, cells were incubated with caspase 3/7 assay solution for 2 hours. The absorbance was measured at 490 nm using a SpectraMax reader (Molecular Devices).</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13507	200 Tests	Price

Citations

View all 11 citations: Citation Explorer

GENERATION OF Usp16 AND Bmi1 KNOCKOUT BREAST CANCER CELL LINES BY CRISPR/Cas9 TECHNOLOGY

Authors:

Garland, Danielle C

Linking pesticide exposure to neurodegenerative diseases: An in vitro investigation with human neuroblastoma cells

Authors:

Alehashem, Maryam and Alcaraz, Alper James and Hogan, Natacha and Weber, Lynn and Siciliano, SD and Hecker, M

Journal:

Science of The Total Environment (2024): 173041

Chrysin Mediates the Induction of Apoptosis in Breast Cancer Cells via the Inhibition of the WNT/$\beta$-Catenin Signaling Pathway

Authors:

{\c{C}}etinkaya, S{\"u}meyra

Folate-Decorated Cross-Linked Cytochrome c Nanoparticles for Active Targeting of Non-Small Cell Lung Carcinoma (NSCLC)

Authors:

Dominguez-Martinez, Irivette and Joaquin-Ovalle, Freisa and Ferrer-Acosta, Yancy and Griebenow, Kai H

Journal:

Pharmaceutics (2022): 490

Cross-Linked Cytochrome c-Based Nanoparticles for Targeted and Controlled Cancer Therapy

Authors:

Martinez, Irivette Dominguez

References

View all 67 references: Citation Explorer

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis

Authors:

Fennell M, Chan H, Wood A.

Journal:

J Biomol Screen (2006): 296

Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells

Authors:

Jiang DJ, Jia SJ, Dai Z, Li YJ.

Journal:

J Mol Cell Cardiol (2006): 529

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	490 nm
Recommended plate	Clear bottom

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Page updated on October 13, 2025

Component A: Caspase 3/7 Substrate (200X Stock Solution)	2 vials (50 µL/vial)
Component B: Assay Buffer	1 bottle (20 mL)