Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Amplite® Colorimetric Caspase 3/7 Assay Kit

Yellow Color

Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color* is a colorimetric assay kit for detecting caspase 3/7 activity in cell extracts and purified enzyme preparations using a DEVD-based chromogenic substrate.

Colorimetric detection: Caspase cleavage of (Z-DEVD)2R110 generates a yellow R110 signal proportional to caspase 3/7 activity.
Sensitive readout: Provides higher sensitivity than conventional DEVD-pNA colorimetric substrates.
Caspase-selective substrate: Uses the DEVD peptide sequence preferred by caspases 3 and 7.
Microplate compatible: Read absorbance at 490 nm using a clear-bottom plate.
Applications: Suitable for apoptosis studies, caspase activity measurements, and drug-screening workflows.

<p>Detection of caspase 3/7 Activity in Jurkat cells. The cells were treated with staurosporine (SS) at the concentration of 0-1 µM for 4 hours at 37ºC. After treatment, cells were incubated with caspase 3/7 assay solution for 2 hours. The absorbance was measured at 490 nm using a SpectraMax reader (Molecular Devices).</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13507	200 Tests	Price

Overview

Caspases play important roles in apoptosis and cell signaling. The activation of Caspase 3/7 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3/7 is also identified as a drug-screening target. Caspase inhibitors have anti-cancer and other pharmalogical potentials. It has been proven that Caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). Our Amplite® Colorimetric Caspase 3/7 Assay Kit uses (Z-DEVD)2R110 as the chromogenic indicator for assaying caspase 3/7 activity. R110 peptide sustrates are colorless. Cleavage of R110 peptides by caspases generates R110, a yellow color dye that can be monitored at 490-520 nm. The increase in the absorbance of caspase-induced R110 is proportional to the activities of caspases. This kit can be used to continuously measure the activities of caspase 3/7 in cell extracts and purified enzyme preparations with an absorbance microplate reader with much higher sensitivity than the other commercial kits that use DEVD-pNA peptide.

Citations

View all 11 citations: Citation Explorer

GENERATION OF Usp16 AND Bmi1 KNOCKOUT BREAST CANCER CELL LINES BY CRISPR/Cas9 TECHNOLOGY

Authors:

Garland, Danielle C

Linking pesticide exposure to neurodegenerative diseases: An in vitro investigation with human neuroblastoma cells

Authors:

Alehashem, Maryam and Alcaraz, Alper James and Hogan, Natacha and Weber, Lynn and Siciliano, SD and Hecker, M

Journal:

Science of The Total Environment (2024): 173041

Chrysin Mediates the Induction of Apoptosis in Breast Cancer Cells via the Inhibition of the WNT/$\beta$-Catenin Signaling Pathway

Authors:

{\c{C}}etinkaya, S{\"u}meyra

Folate-Decorated Cross-Linked Cytochrome c Nanoparticles for Active Targeting of Non-Small Cell Lung Carcinoma (NSCLC)

Authors:

Dominguez-Martinez, Irivette and Joaquin-Ovalle, Freisa and Ferrer-Acosta, Yancy and Griebenow, Kai H

Journal:

Pharmaceutics (2022): 490

Cross-Linked Cytochrome c-Based Nanoparticles for Targeted and Controlled Cancer Therapy

Authors:

Martinez, Irivette Dominguez

References

View all 67 references: Citation Explorer

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis

Authors:

Fennell M, Chan H, Wood A.

Journal:

J Biomol Screen (2006): 296

Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells

Authors:

Jiang DJ, Jia SJ, Dai Z, Li YJ.

Journal:

J Mol Cell Cardiol (2006): 529

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	490 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 29, 2026

Component A: Caspase 3/7 Substrate (200X Stock Solution)	2 vials (50 µL/vial)
Component B: Assay Buffer	1 bottle (20 mL)