Amplite® Colorimetric Enterokinase Activity Assay Kit

Amplite® Colorimetric Enterokinase Activity Assay Kit is a colorimetric assay kit for quantifying enterokinase activity using an enterokinase-specific substrate and EK Yellow™ readout.

Colorimetric detection: Enterokinase-cleaved substrate is detected with EK Yellow™ at 405 nm.
High sensitivity: Detects enterokinase concentrations as low as 3 ng/mL after 30 minutes.
Enzyme-specific substrate: Uses an enterokinase substrate for selective activity measurement.
Microplate compatible: Read absorbance at 405 nm using a clear-bottom plate.
Applications: Suitable for enterokinase activity measurements and enzyme-inhibition studies.

Enterokinase dose response was measured with Amplite® Colorimetric Enterokinase Activity Assay Kit (Cat #11410) on a 96-well clear bottom microplate using a SpectraMax microplate reader (Molecular Devices) with path check on mode.

Protocol

SDS

Catalog	Size	Price	Quantity
11410	100 Tests	Price

Overview

Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system. Enterokinase converts trypsinogen into its active form trypsin, resulting in the subsequent activation of pancreatic digestive enzymes. The deficiency of enterokinase results in intestinal digestion impairment. The inhibition of enterokinase may have anti-tumor effects through suppressing proteases involved in carcinogenesis and metastasis. Therefore, highly selective and sensitive detection of enterokinase plays a key role in biochemical applications. Amplite® Colorimetric Enterokinase Activity Assay Kit offers a sensitive assay for quantifying enterokinase activity. After cleavage by enterokinase, the enterokinase substrate can be detected by EK Yellow™ in an absorbance microplate reader at 405 nm.

References

View all 23 references: Citation Explorer

Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris

Authors:

Melicherova K, Krahulec J, Safranek M, Liskova V, Hopkova D, Szeliova D, Turna J.

Journal:

Appl Microbiol Biotechnol. (2016)

Characterization of a gene family encoding SEA (sea-urchin sperm protein, enterokinase and agrin)-domain proteins with lectin-like and heme-binding properties from Schistosoma japonicum

Authors:

Mbanefo EC, Kikuchi M, Huy NT, Shuaibu MN, Cherif MS, Yu C, Wakao M, Suda Y, Hirayama K.

Journal:

PLoS Negl Trop Dis (2014): e2644

A strategy for fusion expression and preparation of functional glucagon-like peptide-1 (GLP-1) analogue by introducing an enterokinase cleavage site

Authors:

Liu Y, Ren L, Ge L, Cui Q, Cao X, Hou Y, Bai F, Bai G.

Journal:

Biotechnol Lett (2014): 1675

Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox

Authors:

Skala W, Goettig P, Br and stetter H., undefined

Journal:

J Biotechnol (2013): 421

Fusion expression and purification of four disulfide-rich peptides reveals enterokinase secondary cleavage sites in animal toxins

Authors:

Chen Z, Han S, Cao Z, Wu Y, Zhuo R, Li W.

Journal:

Peptides (2013): 145

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	405 nm
Recommended plate	Clear bottom
Instrument specification(s)	Path check on

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Page updated on October 4, 2026

Component A: EK Yellow™	1 vial
Component B: Enterokinase Substrate	1 vial
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Enterokinase Standard	1 vial
Component E: DMSO	1 vial (100 µL)