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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
The Amplite® Colorimetric Fructose-6-Phosphate Assay Kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples. This kit uses a coupled enzyme assay where F6P, in the presence of ATP, is converted to fructose-1,6-diphosphate and ADP by the PFK enzyme. The aldolase enzyme then converts fructose-1,6-diphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Subsequently, glyceraldehyde-3-phosphate dehydrogenase, in the presence of NAD, converts glyceraldehyde-3-phosphate into glyceraldehyde-1,3-bisphosphate and NADH. The NADH produced then reduces a colorless substrate to a colored product (A450 nm), which is proportional to the fructose-6-phosphate present. One unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. Fructose-6-phosphate (F6P) is an important intermediate generated during glycolysis. It is produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. It serves as a precursor to fructose 1,6-bisphosphate. Beyond its role in energy production, F6P can also be shunted to the non-oxidative pentose phosphate pathway as a means of generating pentose phosphates for nucleotide production. F6P levels surge in rapidly proliferating cells like cancer cells, making it a potential metabolic biomarker in diseases.
The dose response of F6P was measured using the Amplite® Colorimetric Fructose-6-Phosphate Assay Kit. The assay was conducted on a 96-well clear bottom microplate, with incubation for 30 minutes, and readings were taken at 450 nm using a ClarioStar microplate reader (BMG).
The dose response of F6P was measured using the Amplite® Colorimetric Fructose-6-Phosphate Assay Kit. The assay was conducted on a 96-well clear bottom microplate, with incubation for 30 minutes, and readings were taken at 450 nm using a ClarioStar microplate reader (BMG).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11330100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance450 nm
Recommended plateClear bottom
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Page updated on March 5, 2026